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dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorSall, Amadou Aen_UK
dc.contributor.authorManuguerra, Jean-Claudeen_UK
dc.contributor.authorKoivogui, Lamineen_UK
dc.contributor.authorAdjami, Aimeen_UK
dc.contributor.authorTraore, Faye Fatouen_UK
dc.contributor.authorHedlund, Kjell-Olafen_UK
dc.contributor.authorLindegren, Gunnelen_UK
dc.contributor.authorMirazimi, Alien_UK
dc.description.abstractInformation on the replication of viral haemorrhagic fever viruses is not readily available and has never been analysed in a comparative approach. Here, we compared the cell culture growth characteristics of haemorrhagic fever viruses (HFV), of the Arenaviridae, Filoviridae, Bunyaviridae, and Flavivridae virus families by performing quantitative analysis of cell culture supernatants by (i) electron microscopy for the quantification of virus particles, (ii) quantitative real time PCR for the quantification of genomes, and (iii) determination of focus forming units by coating fluorescent antibodies to infected cell monolayers for the quantification of virus infectivity. The comparative analysis revealed that filovirus and RVFV replication results in a surplus of genomes but varying degrees of packaging efficiency and infectious particles. More efficient replication and packaging was observed for Lassa virus, and Dengue virus resulting in a better yield of infectious particles while, YFV turned out to be most efficient with only 4 particles inducing one FFU. For Crimean-Congo haemorrhagic fever virus (CCHFV) a surplus of empty shells was observed with only one in 24 particles equipped with a genome. The complete particles turned out to be extraordinarily infectious.en_UK
dc.publisherBioMed Central Ltd.en_UK
dc.relationWeidmann M, Sall AA, Manuguerra J, Koivogui L, Adjami A, Traore FF, Hedlund K, Lindegren G & Mirazimi A (2011) Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures. Virology Journal, 8, Art. No.: 81.
dc.rights© 2011 Weidmann et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_UK
dc.titleQuantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell culturesen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleVirology Journalen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteuren_UK
dc.contributor.affiliationGamal Abdel Nasser University of Conakryen_UK
dc.contributor.affiliationCenters for Disease Control and Prevention (CDC)en_UK
dc.contributor.affiliationMerieux Foundationen_UK
dc.contributor.affiliationSwedish Institute for Infectious Disease Controlen_UK
dc.contributor.affiliationSwedish Institute for Infectious Disease Controlen_UK
dc.contributor.affiliationSwedish Institute for Infectious Disease Controlen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorSall, Amadou A|en_UK
local.rioxx.authorManuguerra, Jean-Claude|en_UK
local.rioxx.authorKoivogui, Lamine|en_UK
local.rioxx.authorAdjami, Aime|en_UK
local.rioxx.authorTraore, Faye Fatou|en_UK
local.rioxx.authorHedlund, Kjell-Olaf|en_UK
local.rioxx.authorLindegren, Gunnel|en_UK
local.rioxx.authorMirazimi, Ali|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameVirology Journal 2011.pdfen_UK
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