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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: One-step RT-PCR for detection of Zika virus
Author(s): Faye, Oumar
Faye, Ousmane
Dupressoir, Anne
Weidmann, Manfred
Ndiaye, Mady
Sall, Amadou A
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Keywords: Zika virus
Issue Date: Sep-2008
Date Deposited: 14-Jan-2014
Citation: Faye O, Faye O, Dupressoir A, Weidmann M, Ndiaye M & Sall AA (2008) One-step RT-PCR for detection of Zika virus. Journal of Clinical Virology, 43 (1), pp. 96-101.
Abstract: Background: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. Objective: To develop a one-step RT-PCR assay to detect ZIKV in human serum. Study design: An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. Results: The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. Conclusions: The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
DOI Link: 10.1016/j.jcv.2008.05.005
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