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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Induction of MxA gene expression by influenza A virus requires type I or type III interferon signaling
Author(s): Holzinger, Dirk
Jorns, Carl
Stertz, Silke
Boisson-Dupuis, Stephanie
Thimme, Robert
Weidmann, Manfred
Casanova, Jean-Laurent
Haller, Otto
Kochs, Georg
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Issue Date: Jul-2007
Date Deposited: 13-Jan-2014
Citation: Holzinger D, Jorns C, Stertz S, Boisson-Dupuis S, Thimme R, Weidmann M, Casanova J, Haller O & Kochs G (2007) Induction of MxA gene expression by influenza A virus requires type I or type III interferon signaling. Journal of Virology, 81 (14), pp. 7776-7785.
Abstract: The human MxA gene belongs to the class of interferon (IFN)-stimulated genes (ISGs) involved in antiviral resistance against influenza viruses. Here, we studied the requirements for MxA induction by influenza A virus infection. MxA is transcriptionally upregulated by type I (alpha and beta) and type III (lambda) IFNs. Therefore, MxA is widely used in gene expression studies as a reliable marker for IFN bioactivity. It is not known, however, whether viruses can directly activate MxA expression in the absence of secreted IFN. By using an NS1-deficient influenza A virus and human cells with defects in IFN production or the STAT1 gene, we studied the induction profile of MxA by real-time reverse transcriptase PCR. The NS1-deficient virus is known to be a strong activator of the IFN system because NS1 acts as a viral IFN-antagonistic protein. Nevertheless, MxA gene expression was not inducible by this virus upon infection of IFN nonproducer cells and STAT1-null cells. Likewise, neither IFN-`alpha` nor IFN-λ had a sizeable effect on the STAT1-null cells, indicating that MxA expression requires STAT1 signaling and cannot be triggered directly by virus infection. In contrast, the expression of the IFN-stimulated gene ISG56 was induced by influenza virus in these cells, confirming that ISG56 differs from MxA in being directly inducible by viral triggers in an IFN-independent way. In summary, our study reveals that MxA is a unique marker for the detection of type I and type III IFN activity during virus infections and IFN therapy.
DOI Link: 10.1128/JVI.00546-06
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