Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/1826
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dc.contributor.authorMorais, Sofiaen_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorZheng, Xiaozhongen_UK
dc.contributor.authorLeaver, Michaelen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2013-06-09T02:44:07Z-
dc.date.available2013-06-09T02:44:07Z-
dc.date.issued2009-10en_UK
dc.identifier.urihttp://hdl.handle.net/1893/1826-
dc.description.abstractFish species vary in their capacity to biosynthesize the n-3 long-chain polyunsaturated fatty acids (LC-PUFA) eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids that are crucial to the health of higher vertebrates. The synthesis of LC-PUFA involves enzyme-mediated fatty acyl desaturation and elongation. Previously, a cDNA for an elongase, now termed elovl5a, had been cloned from Atlantic salmon. Here we report on the cloning of two new elongase cDNAs: a second elovl5b elongase, corresponding to a 294 aa protein, and an elovl2-like elongase, coding for a 287 aa protein, characterized for the first time in a non-mammalian vertebrate. Heterologous expression in yeast showed that the salmon Elovl5b elongated C18 and C20 PUFA, with low activity towards C22, while Elovl2 elongated C20 and C22 PUFA with lower activity towards C18 PUFA. All three transcripts showed predominant expression in the intestine and liver, followed by the brain. Elongase expression showed differential nutritional regulation. Levels of elovl5b and particularly of elovl2, but not of elovl5a, transcripts were significantly increased in liver of salmon fed vegetable oils (VO) compared to fish fed fish oil (FO). Intestinal expression showed a similar pattern. Phylogenetic comparisons indicate that, in contrast to salmon and zebrafish, Acanthopterygian fish species lack elovl2 which is consistent with their neglible ability to biosynthesise LC-PUFA and to adapt to VO dietary inclusion, compared to predominantly freshwater salmonids. Thus the presence of elovl2 in salmon explains the ability of this species to biosynthesise LC-HUFA and may provide a biotechnological tool to produce enhanced levels of LC-PUFA, particularly DHA, in transgenic organisms.en_UK
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.relationMorais S, Monroig O, Zheng X, Leaver M & Tocher DR (2009) Highly Unsaturated Fatty Acid Synthesis in Atlantic Salmon: Characterization of ELOVL5- and ELOVL2-like Elongases. Marine Biotechnology, 11 (5), pp. 627-639. https://doi.org/10.1007/s10126-009-9179-0en_UK
dc.rightsPublished in Marine Biotechnology by Springer.; The original publication is available at www.springerlink.comen_UK
dc.subjectlong-chain polyunsaturated fatty acidsen_UK
dc.subjectbiosynthesisen_UK
dc.subjectfishen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectfatty acyl elongasesen_UK
dc.subjectElovl2en_UK
dc.subjectcDNAen_UK
dc.subjectexpressionen_UK
dc.subjectnutritional regulationen_UK
dc.subjectfish oilen_UK
dc.subjectvegetable oilen_UK
dc.subjectelovl5en_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectFishes Nutritionen_UK
dc.titleHighly Unsaturated Fatty Acid Synthesis in Atlantic Salmon: Characterization of ELOVL5- and ELOVL2-like Elongasesen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1007/s10126-009-9179-0en_UK
dc.citation.jtitleMarine Biotechnologyen_UK
dc.citation.issn1436-2236en_UK
dc.citation.issn1436-2228en_UK
dc.citation.volume11en_UK
dc.citation.issue5en_UK
dc.citation.spage627en_UK
dc.citation.epage639en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000268547600009en_UK
dc.identifier.scopusid2-s2.0-70349452139en_UK
dc.identifier.wtid837806en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.contributor.orcid0000-0002-3155-0844en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2009-10-31en_UK
dc.date.filedepositdate2009-11-26en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMorais, Sofia|en_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorZheng, Xiaozhong|en_UK
local.rioxx.authorLeaver, Michael|0000-0002-3155-0844en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2009-11-26en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2009-11-26|en_UK
local.rioxx.filenameSalmon Elovls Revised.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1436-2228en_UK
Appears in Collections:Aquaculture Journal Articles

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