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dc.contributor.authorDomingo, Cristinaen_UK
dc.contributor.authorPatel, Pranaven_UK
dc.contributor.authorYillah, Jasminen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorMendez, Jairo Aen_UK
dc.contributor.authorNakoune, Emmanuel Ren_UK
dc.contributor.authorNiedrig, Matthiasen_UK
dc.description.abstractReported methods for the detection of the yellow fever viral genome are beset by limitations in sensitivity, specificity, strain detection spectra, and suitability to laboratories with simple infrastructure in areas of endemicity. We describe the development of two different approaches affording sensitive and specific detection of the yellow fever genome: a real-time reverse transcription-quantitative PCR (RT-qPCR) and an isothermal protocol employing the same primer-probe set but based on helicase-dependent amplification technology (RT-tHDA). Both assays were evaluated using yellow fever cell culture supernatants as well as spiked and clinical samples. We demonstrate reliable detection by both assays of different strains of yellow fever virus with improved sensitivity and specificity. The RT-qPCR assay is a powerful tool for reference or diagnostic laboratories with real-time PCR capability, while the isothermal RT-tHDA assay represents a useful alternative to earlier amplification techniques for the molecular diagnosis of yellow fever by field or point-of-care laboratories.en_UK
dc.publisherAmerican Society for Microbiologyen_UK
dc.relationDomingo C, Patel P, Yillah J, Weidmann M, Mendez JA, Nakoune ER & Niedrig M (2012) Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories. Journal of Clinical Microbiology, 50 (12), pp. 4054-4060.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.titleAdvanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratoriesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Journal of Clinical Microbiology 2012.full.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleJournal of Clinical Microbiologyen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNational Institute of Health Colombiaen_UK
dc.contributor.affiliationPasteur Institute of Banguien_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorDomingo, Cristina|en_UK
local.rioxx.authorPatel, Pranav|en_UK
local.rioxx.authorYillah, Jasmin|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorMendez, Jairo A|en_UK
local.rioxx.authorNakoune, Emmanuel R|en_UK
local.rioxx.authorNiedrig, Matthias|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameJournal of Clinical Microbiology 2012.full.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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