Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/17943
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dc.contributor.authorDesbois, Andrew Pen_UK
dc.contributor.authorSattar, Abdulen_UK
dc.contributor.authorGraham, Shirleyen_UK
dc.contributor.authorWarn, Peter Aen_UK
dc.contributor.authorCoote, Peter Jen_UK
dc.date.accessioned2014-08-16T23:09:03Z-
dc.date.available2014-08-16T23:09:03Zen_UK
dc.date.issued2013-11en_UK
dc.identifier.urihttp://hdl.handle.net/1893/17943-
dc.description.abstractObjectives: To evaluate the in vivo effectiveness of a combination treatment containing ranalexin (a natural antimicrobial peptide) and lysostaphin (an antistaphylococcal endopeptidase) for reducing nasal burden of methicillin-resistant Staphylococcus aureus (MRSA). Methods: The community-acquired MRSA strain S. aureus NRS384 (USA300-0114) was used in the present study because it is commonly isolated from human nares and it established consistent and reproducible colonization of cotton rat nares. This model was used to evaluate the efficacy of ranalexin/lysostaphin gels (0.1%-1% w/v; administered intranasally once or once per day for 3 consecutive days) for reducing nasal MRSA burden. Control animals were administered vehicle gel only (0.5% hydroxypropyl methylcellulose) or 2% mupirocin, which is used clinically for nasal decolonization of MRSA. Nasal MRSA burden was assessed at 192 h post-inoculation, which was at least 72 h after the final treatment had been administered. An additional study assessed the efficacy of 0.1% ranalexin/lysostaphin against a mupirocin-resistant MRSA strain (MUP20), which had been selected by serial passage of S. aureus NRS384 through subinhibitory concentrations of mupirocin. Results: Gels containing 0.1% ranalexin/lysostaphin consistently reduced median nasal burden of MRSA to an extent similar to or greater than 2% mupirocin. Treatment with 0.1% ranalexin/lysostaphin was also effective against the MUP20 strain. There was evidence for only minimal irritancy in cotton rat nares administered three doses of 0.1% ranalexin/lysostaphin, suggesting that this agent is suitable for short-course therapy such as is employed currently for nasal decolonization with mupirocin. Conclusions: Ranalexin/lysostaphin could serve as an alternative to mupirocin for nasal decolonization of MRSA.en_UK
dc.language.isoenen_UK
dc.publisherOxford University Pressen_UK
dc.relationDesbois AP, Sattar A, Graham S, Warn PA & Coote PJ (2013) MRSA decolonization of cotton rat nares by a combination treatment comprising lysostaphin and the antimicrobial peptide ranalexin. Journal of Antimicrobial Chemotherapy, 68 (11), pp. 2569-2575. https://doi.org/10.1093/jac/dkt243en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectantibacterialsen_UK
dc.subjectcombinationen_UK
dc.subjectinfection controlen_UK
dc.subjectisoleucyl-tRNA synthetaseen_UK
dc.subjectileSen_UK
dc.subjectmupirocinen_UK
dc.subjectmethicillin-resistant Staphylococcus aureusen_UK
dc.subjectnasal carriageen_UK
dc.titleMRSA decolonization of cotton rat nares by a combination treatment comprising lysostaphin and the antimicrobial peptide ranalexinen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[MRSA decolonization of cotton rat nares.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1093/jac/dkt243en_UK
dc.citation.jtitleJournal of Antimicrobial Chemotherapyen_UK
dc.citation.issn1460-2091en_UK
dc.citation.issn0305-7453en_UK
dc.citation.volume68en_UK
dc.citation.issue11en_UK
dc.citation.spage2569en_UK
dc.citation.epage2575en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailandrew.desbois@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationEuro-protec Ltden_UK
dc.contributor.affiliationUniversity of St Andrewsen_UK
dc.contributor.affiliationEuro-protec Ltden_UK
dc.contributor.affiliationUniversity of St Andrewsen_UK
dc.identifier.isiWOS:000326978600024en_UK
dc.identifier.scopusid2-s2.0-84886257742en_UK
dc.identifier.wtid664549en_UK
dc.contributor.orcid0000-0001-6052-8761en_UK
dcterms.dateAccepted2013-11-30en_UK
dc.date.filedepositdate2013-12-05en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorDesbois, Andrew P|0000-0001-6052-8761en_UK
local.rioxx.authorSattar, Abdul|en_UK
local.rioxx.authorGraham, Shirley|en_UK
local.rioxx.authorWarn, Peter A|en_UK
local.rioxx.authorCoote, Peter J|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameMRSA decolonization of cotton rat nares.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0305-7453en_UK
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