Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/17888
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dc.contributor.authorPreston, Andrew Creeen_UK
dc.contributor.authorTaylor, Johnen_UK
dc.contributor.authorCraig, Brianen_UK
dc.contributor.authorBozzolla, Pierreen_UK
dc.contributor.authorPenman, Daviden_UK
dc.contributor.authorMigaud, Herveen_UK
dc.date.accessioned2014-03-29T23:08:03Z-
dc.date.available2014-03-29T23:08:03Zen_UK
dc.date.issued2013-11en_UK
dc.identifier.urihttp://hdl.handle.net/1893/17888-
dc.description.abstractInland fisheries contribute substantially to the economies of England and Wales. Many trout fisheries rely partly or entirely on stocking to maintain catches. Given that farmed trout often differ genetically from their wild counterparts, wild trout could be at risk of reduced fitness due to interbreeding or competition with farmed fish. As a preventative measure, the UK Environment Agency has implemented the "National Trout and Grayling Strategy" which will only give consent to the stocking of rivers and some still waters with sterile, all-female triploid brown trout. In order to produce an optimised hydrostatic pressure induction protocol three experiments were conducted to (1) determine the optimal timing of application post-fertilisation, (2) define optimal pressure intensity and duration of the shock and (3) study the effect of temperature (6-12 °C) on triploid yield. Triploid rate was assessed using two different ploidy verification methods (blood smear and flow cytometry). Results indicated high survival to yolk sac absorption stage (69.2-93.6%) and high triploid yields (82.5-100%) from the range of treatments applied. Furthermore, no significant differences in triploid rates were shown when shock timings and durations were adjusted according to the temperature used. In all treatments deformity prevalence remained low (< 1.8%) during incubation up to yolk sac absorption (~ 550 degree days post hatch). Overall, this study showed that the optimised pressure shock for the induction of triploidy in brown trout delivering high survival and 100% triploid rate (a prerequisite to brown trout restocking) is a shock with a magnitude of 10,000 psi applied at 300 centigrade temperature minutes (CTM) for at least 5 min duration. The study also validated blood smear and flow cytometry as simple and accurate ploidy assessment techniques for brown trout.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationPreston AC, Taylor J, Craig B, Bozzolla P, Penman D & Migaud H (2013) Optimisation of triploidy induction in brown trout (Salmo trutta L.). Aquaculture, 414-415, pp. 160-166. https://doi.org/10.1016/j.aquaculture.2013.07.034en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectBrown trouten_UK
dc.subjectSalmo trutta Len_UK
dc.subjectTriploiden_UK
dc.subjectPressure shocken_UK
dc.subjectFlow cytometryen_UK
dc.subjectYielden_UK
dc.titleOptimisation of triploidy induction in brown trout (Salmo trutta L.)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[1-s2.0-S0044848613003724-main.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.aquaculture.2013.07.034en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume414-415en_UK
dc.citation.spage160en_UK
dc.citation.epage166en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailherve.migaud@stir.ac.uken_UK
dc.contributor.affiliationMachrihanishen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000326164600020en_UK
dc.identifier.scopusid2-s2.0-84883186864en_UK
dc.identifier.wtid674647en_UK
dc.contributor.orcid0000-0002-3587-5160en_UK
dc.contributor.orcid0000-0003-4370-7922en_UK
dc.contributor.orcid0000-0001-8608-6631en_UK
dc.contributor.orcid0000-0002-5404-7512en_UK
dc.date.accepted2013-07-18en_UK
dcterms.dateAccepted2013-07-18en_UK
dc.date.filedepositdate2013-12-13en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorPreston, Andrew Cree|0000-0002-3587-5160en_UK
local.rioxx.authorTaylor, John|0000-0003-4370-7922en_UK
local.rioxx.authorCraig, Brian|en_UK
local.rioxx.authorBozzolla, Pierre|en_UK
local.rioxx.authorPenman, David|0000-0001-8608-6631en_UK
local.rioxx.authorMigaud, Herve|0000-0002-5404-7512en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filename1-s2.0-S0044848613003724-main.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
Appears in Collections:Aquaculture Journal Articles

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