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dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorHontoria, Franciscoen_UK
dc.contributor.authorNavarro, Juan Carlosen_UK
dc.description.abstractThe meagre, Argyrosomus regius, is a carnivorous fish with great potential to diversify finfish aquaculture in the Mediterranean. However, currently nothing is known about their essential fatty acid requirements. Meagres are marine fish but also display anadromous behaviour migrating to river estuaries to spawn, and thus may provide an insight to the influence of diadromy on biosynthetic ability for long-chain polyunsaturated fatty acid (LC-PUFA). Our primary aim was to characterise key cDNAs (fatty acyl desaturases and elongases) of the biosynthetic pathway as a key step to establish the capacity of meagre for LC-PUFA biosynthesis from shorter chain PUFA. The cDNA sequences of a fatty acyl desaturase (Fads) and elongase of very long-chain fatty acids (Elovl) were obtained using PCR-based methodologies, and function of the proteins was investigated by expression of the coding sequences of the putative desaturase and elongase in the yeast Saccharomyces cerevisiae. The tissue distribution of both cDNAs was studied by reverse transcription PCR. Our results demonstrated that meagre possesses at least one fatty acyl desaturase and one elongase involved in the endogenous production of LC-PUFA. The meagre desaturase and elongase were identified as orthologues of Fads2 and Elovl5, respectively. Functionally, the desaturase had dual Δ6/Δ8 activity, whereas the elongase exhibited high elongation efficiency for C18 and C20 PUFA with low activity towards C22 PUFA. However, we also showed that the meagre Elovl5 elongated 16:3n - 3 to 18:3n - 3, the first time that C16 elongation activity had been demonstrated for a fish elongase. Similar to other marine teleosts, expression of fads2 and elovl5 transcripts was highest in brain. The functions and expression of the meagre Fads2 and Elovl5 proteins suggest that the meagre has a ‘marine type' LC-PUFA biosynthetic pathway, and that its anadromous behaviour has no major influence.en_UK
dc.relationMonroig O, Tocher DR, Hontoria F & Navarro JC (2013) Functional characterisation of a Fads2 fatty acyl desaturase with delta6/delta8 activity and an Elovl5 with C16, C18 and C20 elongase activity in the anadromous teleost meagre (Argyrosomus regius). Aquaculture, 412-413, pp. 14-22.
dc.rightsPublished in Aquaculture by Elsevier; Elsevier believes that individual authors should be able to distribute their accepted author manuscripts for their personal voluntary needs and interests, e.g. posting to their websites or their institution’s repository, e-mailing to colleagues. The Elsevier Policy is as follows: Authors retain the right to use the accepted author manuscript for personal use, internal institutional use and for permitted scholarly posting provided that these are not for purposes of commercial use or systematic distribution. An "accepted author manuscript" is the author’s version of the manuscript of an article that has been accepted for publication and which may include any author-incorporated changes suggested through the processes of submission processing, peer review, and editor-author communications.en_UK
dc.subjectElongase of very long-chain fatty acidsen_UK
dc.subjectEssential fatty acidsen_UK
dc.subjectFatty acyl desaturaseen_UK
dc.subjectPolyunsaturated fatty acidsen_UK
dc.titleFunctional characterisation of a Fads2 fatty acyl desaturase with delta6/delta8 activity and an Elovl5 with C16, C18 and C20 elongase activity in the anadromous teleost meagre (Argyrosomus regius)en_UK
dc.typeJournal Articleen_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorHontoria, Francisco|en_UK
local.rioxx.authorNavarro, Juan Carlos|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameFunctional characterisation of a Fads2 fatty acyl desaturase Final version.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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