Characterization of microsatellite loci in Erysimum mediohispanicum (Brassicaceae) and cross-amplification in related species

Abstract

• Premise of the study : We have developed and optimized microsatellite loci from a genomic library of Erysimum mediohispanicum. Microsatellites were also tested for cross-amplifi cation in 31 other Erysimum species. • Methods and Results : A total of 10 microsatellite loci were successfully amplifi ed. They were polymorphic for 81 E. mediohispanicum individuals from two locations in Sierra Nevada (southeastern Spain), which showed similar patterns of genetic diversity. On average, microsatellites had 8.6 alleles per locus and an expected heterozygosity of 0.69. Only one locus signifi cantly departed from Hardy – Weinberg equilibrium in both locations. Most of the markers successfully amplifi ed in other Erysimum species. • Conclusions : The genetic attributes of microsatellite loci will allow their application to population genetic studies in Erysimum , such as genetic differentiation and structure, gene fl ow, pollinator-mediated speciation, and hybridization studies.