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dc.contributor.authorBussiere, Lucen_UK
dc.contributor.authorDemont, Marcoen_UK
dc.contributor.authorPemberton, Andrew Jen_UK
dc.contributor.authorHall, Matthew Den_UK
dc.contributor.authorWard, Paul Ien_UK
dc.description.abstractIn spite of considerable interest in postcopulatory sexual selection, separating the effects of sperm competition from cryptic female choice remains difficult because mechanisms underlying postcopulatory processes are poorly understood. One methodological challenge is to quantify insemination success for individual males within the sperm stores of multiply mated females to discover how insemination translates into eventual paternity. Any proposed method must be applicable in organisms without extensive DNA sequence information (which include the majority of model species for sexual selection). Here, we describe the development and application of microsatellite competitive-multiplex-PCR for quantifying relative contributions to a small number of sperm in storage. We studied how DNA template characteristics affect PCR amplification of known concentrations of mixed DNA and generated regressions for correcting observations of allelic signal strength based on such characteristics. We used these methods to examine patterns of sperm storage in twice-mated female yellow dung flies, Scathophaga stercoraria. We confirm previous findings supporting sperm displacement and demonstrate that average paternity for the last mate accords with the mean proportion of sperm stored. We further find consistent skew in storage across spermathecae, with more last male sperm stored in the singlet spermatheca on one side of the body than in the doublet on the opposite side. We also show that the time between copulations may be important for effectively sorting sperm. Finally, we demonstrate that male size may influence the opportunity for sperm choice, suggesting future work to disentangle the roles of male competition and cryptic female choice.en_UK
dc.relationBussiere L, Demont M, Pemberton AJ, Hall MD & Ward PI (2010) The assessment of insemination success in yellow dung flies using competitive PCR. Molecular Ecology Resources, 10 (2), pp. 292-303.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectcryptic choiceen_UK
dc.subjectDNA quantificationen_UK
dc.subjectmate choiceen_UK
dc.subjectsperm competitionen_UK
dc.subjectsperm selectionen_UK
dc.subjectDiptera - Evolutionen_UK
dc.titleThe assessment of insemination success in yellow dung flies using competitive PCRen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[The assessment of insemination success in yellow dung flies.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleMolecular Ecology Resourcesen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationUniversity of Munichen_UK
dc.contributor.affiliationUniversity of Zurichen_UK
dc.contributor.affiliationUniversity of New South Walesen_UK
dc.contributor.affiliationUniversity of Zurichen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorBussiere, Luc|0000-0001-8937-8381en_UK
local.rioxx.authorDemont, Marco|en_UK
local.rioxx.authorPemberton, Andrew J|en_UK
local.rioxx.authorHall, Matthew D|en_UK
local.rioxx.authorWard, Paul I|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameThe assessment of insemination success in yellow dung flies.pdfen_UK
Appears in Collections:Biological and Environmental Sciences Journal Articles

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