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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus
Author(s): Scapigliati, Giuseppe
Buonocore, Francesco
Randelli, Elisa
Casani, Daniela
Meloni, Sabrina
Zarletti, Gianpaolo
Tiberi, Mario
Pietretti, D
Boschi, Illenia
Manchado, Manuel
Martin-Antonio, Beatriz
Jimenez-Cantizano, Rosa M
Bovo, Giuseppe
Borghesan, Fabio
Lorenzen, Niels
Einer-Jensen, Katja
Adams, Alexandra
Thompson, Kimberly
Alonso, Carlos
Bejar, Julia
Cano, Irene
Borrego, Juan J
Alvarez, M Carmen
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Keywords: Sea bass Dicentrarchus labrax
Betanodavirus VER
PCR array
Anti-viral immune defence
Sea basses Diseases
European seabass
Issue Date: Feb-2010
Date Deposited: 12-Jun-2013
Citation: Scapigliati G, Buonocore F, Randelli E, Casani D, Meloni S, Zarletti G, Tiberi M, Pietretti D, Boschi I, Manchado M, Martin-Antonio B, Jimenez-Cantizano RM, Bovo G, Borghesan F, Lorenzen N, Einer-Jensen K, Adams A, Thompson K, Alonso C, Bejar J, Cano I, Borrego JJ & Alvarez MC (2010) Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. Fish and Shellfish Immunology, 28 (2), pp. 303-311.
Abstract: Naïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable "in vitro" increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.
DOI Link: 10.1016/j.fsi.2009.11.008
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