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dc.contributor.authorLopez, Jimena Benjamin-
dc.contributor.authorAlonso, Maria del Carmen-
dc.contributor.authorThompson, Kimberly-
dc.contributor.authorAdams, Alexandra-
dc.contributor.authorInfante, Carlos-
dc.contributor.authorCastro, Dolores-
dc.contributor.authorBorrego, Juan J-
dc.contributor.authorGarcia-Rosado, Esther-
dc.description.abstractThe distribution of viral genome in the tissues of juvenile European seabass (Dicentrarchus labrax) during the course of a Red Spotted Grouper Nervous Necrosis Virus (RGNNV) infection has not yet been described. The present study addresses this and indicates which target organs may be involved in viral replication. This information should enable more accurate detection of virus in asymptomatic carriers, and in turn help to control the spread of the disease. The aim of this study was to examine the pattern of expression of viral genomic segments RNA1 and RNA2, using two absolute real-time PCRs (RT-qPCR), over the course of a RGNNV infection after administering the virus by intramuscular injection. In situ hybridization was also used to locate the RNA2 viral segment in different organs throughout the infection. The experimental challenge provoked an acute form of viral nervous necrosis (VNN), with a resulting cumulative mortality of 37%. The RT-qPCRs designed allowed the detection of both genomic segments in all the organs tested (nervous and non-nervous tissues) at all sampling times examined. The highest viral RNA copy number was found in eyes, although viral replication appeared to begin in the brain. Viral replication was also recorded in pooled internal organs and in caudal fin. However, the increase in the viral RNA copy number in these organs did not result in an increased viral titre, which may indicate that a productive infection does not take place in non-nervous tissues, possibly due to a failure in a viral post-replication step.en_UK
dc.relationLopez Jimena B, Alonso MdC, Thompson K, Adams A, Infante C, Castro D, Borrego JJ & Garcia-Rosado E (2011) Tissue distribution of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) genome in experimentally infected juvenile European seabass (Dicentrarchus labrax), Veterinary Microbiology, 154 (1-2), pp. 86-95.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectEuropean seabassen_UK
dc.subjectRGNNV genotypeen_UK
dc.subjectTissue distributionen_UK
dc.subjectQuantitative real-time PCRen_UK
dc.subjectIn situ hybridizationen_UK
dc.subject.lcshEuropean seabass fisheries-
dc.subject.lcshEuropean seabass.-
dc.titleTissue distribution of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) genome in experimentally infected juvenile European seabass (Dicentrarchus labrax)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleVeterinary Microbiology-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationUniversity of Malaga-
dc.contributor.affiliationCentro IFAPA, Spain-
dc.contributor.affiliationUniversity of Malaga-
dc.contributor.affiliationUniversity of Malaga-
dc.contributor.affiliationUniversity of Malaga-
Appears in Collections:Aquaculture Journal Articles

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