Background Much has been made of the potential for aquaculture to improve rural livelihoods and food and nutrition security in Africa, though little evidence exists to back such claims. This study, conducted in northern Zambia, assessed the benefits of adopting aquaculture by comparing a sample of households with (n = 177) and without fishponds (n = 174). Results On-farm food production was assessed by summing all crop and livestock activities and calculating a production diversity score (PD...
Disease and parasitism cause major welfare, environmental and economic concerns for global aquaculture. In this review, we examine the status and potential of technologies that exploit genetic variation in host resistance to tackle this problem. We argue that there is an urgent need to improve understanding of the genetic mechanisms involved, leading to the development of tools that can be applied to boost host resistance and reduce the disease burden. We draw on two pressing global disease p...
The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei ...
The liver is a multitasking organ with essential functions for vertebrate health spanning metabolism and immunity. In contrast to mammals, our understanding of liver cellular heterogeneity and its role in regulating immunological status remains poorly defined in fishes. Addressing this knowledge gap, we generated a transcriptomic atlas of 47,432 nuclei isolated from the liver of Atlantic salmon (Salmo salar L.) contrasting control fish with those challenged with a pathogenic strain of Aeromon...
This study is the first to investigate the effects of post-ovulatory ageing (POA) on both diploid and triploid Atlantic salmon (Salmo salar L.). Following ovulation, female broodstock were partially stripped periodically (at 0, 5, 10, 15 and 20 days post-ovulation, DPO), creating five groups of ova with differing durations held in the body cavity. Survival and bone architecture were negatively impacted by increasing POA, irrespective of ploidy, and an increase in individual growth variation w...
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