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dc.contributor.authorOliver, Margaret-
dc.contributor.authorSkuce, Phillip-
dc.contributor.authorMcNair, Carol-
dc.contributor.authorKnox, David-
dc.description.abstractAsparaginyl proteinases (or legumains) are a recently identified, novel class of cysteine proteinase which specifically hydrolyse peptide bonds after asparagine residues. Legumains have been implicated in the activation of cysteine proteases, particularly cathepsin B-like proteinases which are thought to help degrade the bloodmeal in blood-feeding helminths such as schistosomes, hookworms and other nematode species. An EST sequence representing a full-length legumain was identified from the Haemonchus contortus dataset 1 . This encoded a protein with a predicted Mr of 49 kDa, the amino acid sequence of which showed good homology (34-40% identity) to legumains from Schistosoma mansoni, human and rat and contained a legumain-like active site. RT-PCR indicated that the legumain transcript was expressed from the L4 life-cycle stage onwards. The coding sequence was expressed in E. coli and antibodies to the resultant recombinant protein indicated that the enzyme was expressed in the microvillar surface of the intestinal cells. Legumain activity was detected in extracts of the adult parasite but not the host protective Thiol-Sepharose-binding fraction, although it was detectable in the latter by immunoblot. Activity was relatively insensitive to E64, an inhibitor of cysteine proteinases and completely inhibited by the alkylating agent, N-ethylmaleimide, consistent with inhibitor effects on previously characterized legumains.en_UK
dc.publisherCambridge University Press-
dc.relationOliver M, Skuce P, McNair C & Knox D (2006) Identification and characterization of an asparaginyl proteinase (legumain) from the parasitic nematode, Haemonchus contortus, Parasitology, 133 (2), pp. 237-244.-
dc.rightsThe publisher has not responded to our queries therefore this work cannot be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectHaemonchus contortusen_UK
dc.subjectenzyme activityen_UK
dc.titleIdentification and characterization of an asparaginyl proteinase (legumain) from the parasitic nematode, Haemonchus contortusen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher has not responded to our queries. This work cannot be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationThe Moredun Research Institute-
dc.contributor.affiliationThe Moredun Research Institute-
dc.contributor.affiliationThe Moredun Research Institute-
Appears in Collections:Aquaculture Journal Articles

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