STORRE
STORRE: Stirling Online Research Repository
http://hdl.handle.net/1893/10491| Appears in Collections: | Biological and Environmental Sciences Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Effects of known phenoloxidase inhibitors on hemocyanin-derived phenoloxidase from Limulus polyphemus |
| Author(s): | Wright, Jamie Clark, William McCaskill Cairn, Jennifer A Patterson, Alan Coates, Christopher Nairn, Jacqueline |
| Contact Email: | jn2@stir.ac.uk |
| Keywords: | Hemocyanin Phenoloxidase Hemocyanin-derived phenoloxidase Kinetics Inhibition |
| Issue Date: | Nov-2012 |
| Date Deposited: | 16-Jan-2013 |
| Citation: | Wright J, Clark WM, Cairn JA, Patterson A, Coates C & Nairn J (2012) Effects of known phenoloxidase inhibitors on hemocyanin-derived phenoloxidase from Limulus polyphemus. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 163 (3-4), pp. 303-308. https://doi.org/10.1016/j.cbpb.2012.07.003 |
| Abstract: | Inhibitors of phenoloxidase are used routinely to characterise the structural and functional properties of phenoloxidases. Hemocyanin-derived phenoloxidase activity is also sensitive to standard phenoloxidase inhibitors. In this study, we characterise the effects of a number of phenoloxidase inhibitors on hemocyanin-derived phenoloxidase activity from the chelicerate, Limulus polyphemus. Both inhibition type and Ki values were similar to those observed for hemocyanin-derived phenoloxidase from another chelicerate, Eurypelma californicum. In addition, substrate inhibition was observed at concentrations above 2 mM dopamine. The conformation in which two of the inhibitors, namely tropolone and kojic acid, would bind near the Cu(II) centre of hemocyanin is proposed. |
| DOI Link: | 10.1016/j.cbpb.2012.07.003 |
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| Licence URL(s): | http://www.rioxx.net/licenses/under-embargo-all-rights-reserved |
| File | Description | Size | Format | |
|---|---|---|---|---|
| Nairn4.pdf | Fulltext - Published Version | 733.03 kB | Adobe PDF | Under Permanent Embargo Request a copy |
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