Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/10244
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Suppression of first cleavage in the Nile tilapia, Oreochromis niloticus L. -- a comparison of the relative effectiveness of pressure and heat shocks
Author(s): Hussain, M Gulam
Penman, David
McAndrew, Brendan
Johnstone, Ray
Contact Email: b.j.mcandrew@stir.ac.uk
Issue Date: Apr-1993
Date Deposited: 12-Nov-2012
Citation: Hussain MG, Penman D, McAndrew B & Johnstone R (1993) Suppression of first cleavage in the Nile tilapia, Oreochromis niloticus L. -- a comparison of the relative effectiveness of pressure and heat shocks. Aquaculture, 111 (1-4), pp. 263-270. https://doi.org/10.1016/0044-8486%2893%2990043-X
Abstract: Evidence is presented for the successful suppression of first cleavage in Oreochromis niloticus L. and thereby the production of mitotic gynogens. The optimal parameters for UV irradiation of milt were 300-310 μW cm-2 for 2 min at 4°C. The optimal pressure shock parameters were 630 kg cm-2 (9000 psi) for 2 min at 28°C at 40-50 min after fertilization (AF) and those for heat shock were 41°C for 3.5 min at 27.5-30 min AF. Isozyme analysis of survivors at two loci, ADA∗ and EST-2∗, confirmed homozygosity and lack of paternal inheritance.
DOI Link: 10.1016/0044-8486(93)90043-X
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