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dc.contributor.authorShinn, Andrewen_UK
dc.contributor.authorBanks, Bryonyen_UK
dc.contributor.authorTange, Nahokoen_UK
dc.contributor.authorBron, Jamesen_UK
dc.contributor.authorSommerville, Christinaen_UK
dc.contributor.authorAoki, Takashien_UK
dc.contributor.authorWootten, Rodneyen_UK
dc.description.abstractGenetic differentiation within the salmon louse Lepeophtheirus salmonis (Krøyer, 1837), was investigated by the sequencing of specific nucleotide regions. Partial sequences of the 18S ribosomal RNA gene and the ribosomal internal transcribed spacer (ITS-1) region from single sea lice were amplified by the polymerase chain reaction (PCR). Lice were collected from wild and farmed Atlantic salmon (Salmo salar L., 1758) from nine selected localities around the Scottish coastline. A 0.9kb fragment of the 18S ribosomal RNA gene was amplified and compared for several samples of lice which showed no observable differences between the lice from different collection sites confirming the absence of cryptic species. The 454 nucleotide ITS-1 sequence showed differences between derived sequences from 13 sea lice samples from 4 collection sites which included 2 farm sites and 2 sites where lice were taken from wild fish. Across all samples, there was a 92.14% similarity in the ITS-1 sequence. The percentage similarity in the ITS-1 sequence in samples of lice from two fish farms were 99.71% (site A) and 95.72% (site D) but only 86.90% (site B) and 86.03% (site C) similarity was shown in lice samples taken from sites where wild salmonids were caught. The greater similarity between the ITS-1 sequence within farm sites may be attributed to a restricted gene flow within lice populations in Atlantic salmon cage sites.en_UK
dc.relationShinn A, Banks B, Tange N, Bron J, Sommerville C, Aoki T & Wootten R (2000) Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland. Contributions to Zoology, 69 (1/2), pp. 89-98.
dc.rightsPublisher is open-access. Open access publishing allows free access to and distribution of published articles where the author retains copyright of their work by employing a Creative Commons attribution 3.0 License. Proper attribution of authorship and correct citation details should be given.en_UK
dc.subjectSea louseen_UK
dc.subjectLepeophtheirus salmonisen_UK
dc.subjectAtlantic salmonen_UK
dc.subject18S ribosomal DNAen_UK
dc.subjectinternal transcribed spaceren_UK
dc.subjectpopulation geneticsen_UK
dc.titleUtility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotlanden_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleContributions to Zoologyen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationTokyo University of Fisheriesen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationTokyo University of Fisheriesen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorShinn, Andrew|0000-0002-5434-2685en_UK
local.rioxx.authorBanks, Bryony|en_UK
local.rioxx.authorTange, Nahoko|en_UK
local.rioxx.authorBron, James|0000-0003-3544-0519en_UK
local.rioxx.authorSommerville, Christina|en_UK
local.rioxx.authorAoki, Takashi|en_UK
local.rioxx.authorWootten, Rodney|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameShinn et al 2000a 18S sequencing.pdfen_UK
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