Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCoward, Kevin-
dc.contributor.authorCampos-Mendoza, Antonio-
dc.contributor.authorLarman, Mark-
dc.contributor.authorHibbitt, Olivia-
dc.contributor.authorMcAndrew, Brendan-
dc.contributor.authorBromage, Niall R-
dc.contributor.authorParrington, John-
dc.description.abstractEstablished studies in a variety of organisms including amphibians, fish, ascidians, nemerteans, echinoderms, mammals, and even a species of flowering plant, clearly demonstrate that an increase in intracellular egg calcium is crucial to the process of egg activation at fertilization. In echinoderms, egg activation appears to involve an egg phospholipase C gamma (PLCγ). However, numerous studies in mammalian species suggest that calcium is released from internal egg stores at fertilization by a sperm-derived cytosolic protein factor. Recent studies in the mouse have identified this sperm-derived factor as being a novel sperm-specific PLC isoform with distinctive properties (PLCζ). Homologues of PLCζ have since been isolated from human and cynomolgus monkey sperm. In addition, sperm factor activity has been detected in non-mammalian species such as chicken, Xenopus, and a flowering plant. Here we report evidence for the existence of a similar sperm-derived factor in a commercially important species of teleost fish, the Nile tilapia Oreochromis niloticus (L). Using an established bioassay for calcium release, the sea urchin egg homogenate, we demonstrate that protein extracts obtained from tilapia spermatozoa exhibit PLC activity similar to that seen in mammalian sperm extracts, and also induce calcium release when added directly to the homogenate. Further, tilapia sperm extracts induced calcium oscillations when injected into mouse oocytes.en_UK
dc.relationCoward K, Campos-Mendoza A, Larman M, Hibbitt O, McAndrew B, Bromage NR & Parrington J (2003) Teleost fish spermatozoa contain a cytosolic protein factor that induces calcium release in sea urchin egg homogenates and triggers calcium oscillations when injected into mouse oocytes, Biochemical and Biophysical Research Communications, 305 (2), pp. 299-304.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectPLC xien_UK
dc.subjectegg activationen_UK
dc.subject.lcshFishes Spermatozoa-
dc.subject.lcshNile tilapia-
dc.titleTeleost fish spermatozoa contain a cytosolic protein factor that induces calcium release in sea urchin egg homogenates and triggers calcium oscillations when injected into mouse oocytesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleBiochemical and Biophysical Research Communications-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationUniversity of Oxford-
dc.contributor.affiliationMichoacan University of San Nicolas de Hildalgo (UMSNH)-
dc.contributor.affiliationUniversity College London-
dc.contributor.affiliationUniversity of Oxford-
dc.contributor.affiliationUniversity of Stirling-
dc.contributor.affiliationUniversity of Oxford-
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
coward_BBRC_2003.pdf155.54 kBAdobe PDFUnder Permanent Embargo    Request a copy

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.