|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Induced gynogenesis in the silver barb (Puntius gonionotus Bleeker) and evidence for female homogamety|
|Citation:||Pongthana N, Penman D, Karnasuta J & McAndrew B (1995) Induced gynogenesis in the silver barb (Puntius gonionotus Bleeker) and evidence for female homogamety. Aquaculture, 135 (4), pp. 267-276. https://doi.org/10.1016/0044-8486%2895%2901033-5|
|Abstract:||Diploid gynogenesis was induced experimentally in the silver barb (Puntius gonionotus) as the first step in a programme aimed at monosex female production. Sperm was exposed to ultraviolet light at a dose rate of 196 μWcm-2 for 0-32 min (0-376.3 mJcm-2), using a standard sperm concentration of 8 × 108ml-1. The optimal UV dose for producing haploid gynogenetics when such irradiated sperm was used to fertilise eggs was 1 min (11.8 mJcm-2). Diploidisation of the gynogenetic genome was achieved via temperature shocks. Cold shocks of 2 ± 0.1 °C for 10, 20 or 30 min or a heat shock of 40 ± 0.1 °C for 1.0 min were applied at various times after fertilisation (pre-shock incubation temperature 29 ± 1 °C). A maximum survival rate of 61.3% viable diploid gynogenetic offspring (relative to control) was obtained using a cold shock of 2 ± 0.1 °C for 10 min starting 1.5 min after fertilisation. A total of 268 diploid gynogenetic fry from ten different females were reared and sexed by histological examination of the gonads. With the exception of four undifferentiated fish, all of the gynogenetic fish were female, in contrast to the controls, which had a mean sex ratio of 54.6% female (which was not significantly different from 50% female). The implications of these results for monosex female production are discussed.|
|Rights:||The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.|
|pongthanaetal_Aquaculture_1995.pdf||Fulltext - Published Version||888.18 kB||Adobe PDF||Under Embargo until 2999-12-16 Request a copy|
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/
If you believe that any material held in STORRE infringes copyright, please contact email@example.com providing details and we will remove the Work from public display in STORRE and investigate your claim.