Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9961
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: A molecular study of Eubothrium rugosum (Batsch, 1786) (Cestoda: Pseudophyllidea) using ITS rDNA sequences, with notes on the distribution and intraspecific sequence variation of Eubothrium crassum (Bloch, 1779)
Authors: Kralova-Hromadova, Iveta
Scholz, Tomas
Shinn, Andrew
Cunningham, Carey
Wootten, Rodney
Hanzelova, Vladimira
Sommerville, Christina
Contact Email: aps1@stir.ac.uk
Issue Date: Apr-2003
Publisher: Springer
Citation: Kralova-Hromadova I, Scholz T, Shinn A, Cunningham C, Wootten R, Hanzelova V & Sommerville C (2003) A molecular study of Eubothrium rugosum (Batsch, 1786) (Cestoda: Pseudophyllidea) using ITS rDNA sequences, with notes on the distribution and intraspecific sequence variation of Eubothrium crassum (Bloch, 1779), Parasitology Research, 89 (6), pp. 473-479.
Abstract: The internal transcribed spacers (ITS-1 and ITS-2) of the ribosomal DNA (rDNA) of fish cestodes of the genus Eubothrium were sequenced. The ITS sequences of Eubothrium rugosum were determined and compared with previously analysed congeneric species, Eubothrium crassum and Eubothrium salvelini. The ITS-1 sequences of E. rugosum and E. crassum were 535 bp long, the length of E. salvelini ITS-1 was 536 bp. The ITS-2 region was found to be 403 bp in E. rugosum and E. crassum, and 401-402 bp in E. salvelini. The ITS-1 region of E. rugosum was closer to E. salvelini (identity 98.9-99.1%) than to E. crassum (97.9-98.1%), while the sequence similarity within the ITS-2 region was almost identical (97.5-98.0% for E. crassum; 97.7-98.3% for E. salvelini). Several restriction enzymes were found to be suitable for the differentiation of the three Eubothrium species by PCR-RFLP. The intraspecific sequence variation of E. crassum from different fish hosts (freshwater and marine) and European regions was very low, 0.2% for ITS-1 and 0.5% for ITS-2. Analysis of the ITS sequences of specimens from rainbow trout from three localities in Scotland revealed that both E. crassum and E. salvelini are present in this fish host.
Type: Journal Article
URI: http://hdl.handle.net/1893/9961
URL: http://link.springer.com/article/10.1007/s00436-002-0779-0
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Slovak Academy of Sciences
Czech Academy of Sciences
Aquaculture
FRS Marine Laboratory
Aquaculture
Slovak Academy of Sciences
Aquaculture

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