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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9961
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dc.contributor.authorKralova-Hromadova, Ivetaen_UK
dc.contributor.authorScholz, Tomasen_UK
dc.contributor.authorShinn, Andrewen_UK
dc.contributor.authorCunningham, Careyen_UK
dc.contributor.authorWootten, Rodneyen_UK
dc.contributor.authorHanzelova, Vladimiraen_UK
dc.contributor.authorSommerville, Christinaen_UK
dc.date.accessioned2012-11-20T23:15:09Z-
dc.date.available2012-11-20T23:15:09Zen_UK
dc.date.issued2003-04en_UK
dc.identifier.urihttp://hdl.handle.net/1893/9961-
dc.description.abstractThe internal transcribed spacers (ITS-1 and ITS-2) of the ribosomal DNA (rDNA) of fish cestodes of the genus Eubothrium were sequenced. The ITS sequences of Eubothrium rugosum were determined and compared with previously analysed congeneric species, Eubothrium crassum and Eubothrium salvelini. The ITS-1 sequences of E. rugosum and E. crassum were 535 bp long, the length of E. salvelini ITS-1 was 536 bp. The ITS-2 region was found to be 403 bp in E. rugosum and E. crassum, and 401-402 bp in E. salvelini. The ITS-1 region of E. rugosum was closer to E. salvelini (identity 98.9-99.1%) than to E. crassum (97.9-98.1%), while the sequence similarity within the ITS-2 region was almost identical (97.5-98.0% for E. crassum; 97.7-98.3% for E. salvelini). Several restriction enzymes were found to be suitable for the differentiation of the three Eubothrium species by PCR-RFLP. The intraspecific sequence variation of E. crassum from different fish hosts (freshwater and marine) and European regions was very low, 0.2% for ITS-1 and 0.5% for ITS-2. Analysis of the ITS sequences of specimens from rainbow trout from three localities in Scotland revealed that both E. crassum and E. salvelini are present in this fish host.en_UK
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.relationKralova-Hromadova I, Scholz T, Shinn A, Cunningham C, Wootten R, Hanzelova V & Sommerville C (2003) A molecular study of Eubothrium rugosum (Batsch, 1786) (Cestoda: Pseudophyllidea) using ITS rDNA sequences, with notes on the distribution and intraspecific sequence variation of Eubothrium crassum (Bloch, 1779). Parasitology Research, 89 (6), pp. 473-479. http://link.springer.com/article/10.1007/s00436-002-0779-0en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.titleA molecular study of Eubothrium rugosum (Batsch, 1786) (Cestoda: Pseudophyllidea) using ITS rDNA sequences, with notes on the distribution and intraspecific sequence variation of Eubothrium crassum (Bloch, 1779)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[Kralova-Hromadova et al 2003 Parasitol Res 2003.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleParasitology Researchen_UK
dc.citation.issn1432-1955en_UK
dc.citation.issn0932-0113en_UK
dc.citation.volume89en_UK
dc.citation.issue6en_UK
dc.citation.spage473en_UK
dc.citation.epage479en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.identifier.urlhttp://link.springer.com/article/10.1007/s00436-002-0779-0en_UK
dc.author.emailaps1@stir.ac.uken_UK
dc.contributor.affiliationSlovak Academy of Sciencesen_UK
dc.contributor.affiliationCzech Academy of Sciencesen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationFRS Marine Laboratoryen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationSlovak Academy of Sciencesen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.scopusid2-s2.0-0346037258en_UK
dc.identifier.wtid756180en_UK
dc.contributor.orcid0000-0002-5434-2685en_UK
dcterms.dateAccepted2003-04-30en_UK
dc.date.filedepositdate2012-11-19en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorKralova-Hromadova, Iveta|en_UK
local.rioxx.authorScholz, Tomas|en_UK
local.rioxx.authorShinn, Andrew|0000-0002-5434-2685en_UK
local.rioxx.authorCunningham, Carey|en_UK
local.rioxx.authorWootten, Rodney|en_UK
local.rioxx.authorHanzelova, Vladimira|en_UK
local.rioxx.authorSommerville, Christina|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameKralova-Hromadova et al 2003 Parasitol Res 2003.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0932-0113en_UK
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