Please use this identifier to cite or link to this item:
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: 3D HCCH3 -TOCSY for resonance assignment of methyl-containing side chains in 13C-labeled proteins
Authors: Uhrin, Dusan
Uhrinova, Stanislava
Leadbeater, Claire
Nairn, Jacqueline
Price, Nicholas C
Barlow, Paul N
Contact Email:
Keywords: 3D NMR
phosphoglycerate mutase
protein side chains
methyl-containing amino acids
Issue Date: Feb-2000
Publisher: Elsevier
Citation: Uhrin D, Uhrinova S, Leadbeater C, Nairn J, Price NC & Barlow PN (2000) 3D HCCH3 -TOCSY for resonance assignment of methyl-containing side chains in 13C-labeled proteins, Journal of Magnetic Resonance, 142 (2), pp. 288-293.
Abstract: Two 3D experiments, (H)CCH3-TOCSY and H(C)CH3-TOCSY, are proposed for resonance assignment of methyl-containing amino acid side chains. After the initial proton–carbon INEPT step, during which either carbon or proton chemical shift labeling is achieved (t1), the magnetization is spread along the amino acid side chains by a carbon spin lock. The chemical shifts of methyl carbons are labeled (t2) during the following constant time interval. Finally the magnetization is transferred, in a reversed INEPT step, to methyl protons for detection (t3). The proposed experiments are characterized by high digital resolution in the methyl carbon dimension (t2max = 28.6 ms), optimum sensitivity due to the use of proton decoupling during the long constant time interval, and an optional removal of CH2, or CH2 and CH, resonances from the F2F3 planes. The building blocks used in these experiments can be implemented in a range of heteronuclear experiments focusing on methyl resonances in proteins. The techniques are illustrated using a 15N, 13C-labeled E93D mutant of Schizosacharomyces pombe phosphoglycerate mutase (23.7 kDa).
Type: Journal Article
DOI Link:
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Edinburgh
University of Edinburgh
University of Edinburgh
Biological and Environmental Sciences
University of Stirling
University of Edinburgh

Files in This Item:
File Description SizeFormat 
Uhrin etl al.pdf129.57 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.