|Appears in Collections:||Biological and Environmental Sciences Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Thirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related species|
Solanum grayi var. grayi
Solanum grayi var. grandiflorum
Solanum sect. Androceras
|Citation:||Vallejo-Marin M, Solis-Montero L, Bacles C & Lepais O (2011) Thirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related species, American Journal of Botany, 98 (10), pp. e296- e299.|
|Abstract:||Premise of the study: Microsatellite markers were developed using second- generation sequencing in Solanum rostratum as a tool to study the reproductive biology and genetic structure of this invasive species. Methods and Results: Thirteen microsatellites were successfully discovered and amplified in a single multiplexed PCR. All loci showed genetic variation in S. rostratum. Cross-amplification in five closely related taxa was successful for a subset of loci. Conclusions: The set of 13 microsatellite markers developed here provides a time-effective and cost-effective genetic tool to study the reproductive biology of S. rostratum. The demonstrated transferability of the PCR multiplex to related taxa also highlights its usefulness for evolutionary studies across Solanum sect. Androceras.|
|Rights:||Publisher policy allows this work to be made available in this repository. Published in American Journal of Botany by Botanical Society of America . The original publication is available at http://www.amjbot.org/content/98/10/e296|
|Vallejo-Marin et al_Solanum microsatellites_Revision 2.pdf||188.5 kB||Adobe PDF||View/Open|
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