Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7566
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles
Peer Review Status: Refereed
Title: Leg glucose and protein metabolism during an acute bout of resistance exercise in humans
Authors: Durham, William J
Miller, Sharon L
Yeckel, Catherine W
Chinkes, David L
Tipton, Kevin
Rasmussen, Blake B
Wolfe, Robert R
Contact Email: k.d.tipton@stir.ac.uk
Keywords: rate of appearance
rate of disappearance
Issue Date: Oct-2004
Publisher: The American Physiological Society
Citation: Durham WJ, Miller SL, Yeckel CW, Chinkes DL, Tipton K, Rasmussen BB & Wolfe RR (2004) Leg glucose and protein metabolism during an acute bout of resistance exercise in humans, Journal of Applied Physiology, 97 (4), pp. 1379-1386.
Abstract: The present study investigated the responses of leg glucose and protein metabolism during an acute bout of resistance exercise. Seven subjects (5 men, 2 women) were studied at rest and during a strenuous lower body resistance exercise regimen consisting of ∼8 sets of 10 repetitions of leg press at ∼75% 1 repetition maximum and 8 sets of 8 repetitions of knee extensions at ∼80% 1 repetition maximum. l-[ring-2H5]phenylalanine was infused throughout the study for measurement of phenylalanine rates of appearance, disappearance, protein synthesis, and protein breakdown across the leg. Femoral arterial and venous blood samples were collected at rest and during exercise for determination of leg blood flow, concentrations of glucose, lactate, alanine, glutamine, glutamate, leucine, and phenylalanine, and phenylalanine enrichments. Muscle biopsies were obtained at rest and immediately after exercise. Leg blood flow was nearly three times (P less than 0.009) higher and glucose uptake more than five times higher (P = 0.009) during exercise than at rest. Leg lactate release was 86 times higher than rest during the exercise bout. Although whole body phenylalanine rate of appearance, an indicator of whole body protein breakdown, was reduced during exercise; leg phenylalanine rate of appearance, rate of disappearance, protein synthesis, and protein breakdown did not change. Arterial and venous alanine concentrations and glutamate uptake were significantly higher during exercise than at rest. We conclude that lower body resistance exercise potently stimulates leg glucose uptake and lactate release. In addition, muscle protein synthesis is not elevated during a bout of resistance exercise.
Type: Journal Article
URI: http://hdl.handle.net/1893/7566
URL: http://jap.physiology.org/content/97/4/1379
DOI Link: http://dx.doi.org/10.1152/japplphysiol.00635.2003
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Texas
University of Texas Medical Branch
University of Texas
University of Texas
Sport
University of Texas Medical Branch
University of Texas

Files in This Item:
File Description SizeFormat 
1379.full.pdf131.34 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.