Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7472
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Mitochondrial DNA mutation frequencies in experimentally irradiated compost worms, Eisenia fetida
Authors: Wilding, Craig S
Trikic, Michael Z
Hingston, Joanne L
Copplestone, David
Tawn, E Janet
Contact Email: david.copplestone@stir.ac.uk
Keywords: annelid
biomarker
compost worm
ionising radiation
mitochondrial DNA
mutation
Issue Date: 31-Jan-2006
Publisher: Elsevier
Citation: Wilding CS, Trikic MZ, Hingston JL, Copplestone D & Tawn EJ (2006) Mitochondrial DNA mutation frequencies in experimentally irradiated compost worms, Eisenia fetida, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 603 (1), pp. 56-63.
Abstract: The compost worm Eisenia fetida is routinely used in ecotoxicological studies. A standard assay to assess genetic damage in this species would be extremely valuable. Since mitochondrial DNA (mtDNA) is known to exhibit an increased mutation rate following exposure to ionising radiation we assessed the validity of a mtDNA-based assay for measuring increases in mutation rate in laboratory- irradiated compost worms. To this end the mutation frequency in the mtDNA of the compost worm E. fetida was quantified following in vivo γ-irradiation of adult worms in three dose groups. Five adult worms exposed to 1.4 mGy/h for 55 days (total dose 1.85 Gy), five adult worms exposed to 8.5 mGy/h for 55 days (total dose 11.22 Gy) and five adult control worms were used to assess the effect of irradiation on mtDNA mutation induction. DNA samples extracted from irradiated adult worms were used in high-fidelity PCR of a 486 bp region of mtDNA spanning the ATPase 8 gene, chosen for its high spontaneous mutation rate. PCR products were cloned and sequenced to identify mutations, with 89–102 clones successfully sequenced per individual. A significant elevation in mtDNA mutation frequency (p = 0.032) was seen in worms exposed at the higher dose rate (8.5 mGy/h, total dose 11.22 Gy; mutation frequency 27.98 ± 4.85 × 10−5 mutations/bp) in comparison to controls (mutation frequency 12.68 ± 3.06 × 10−5 mutations/bp), but no elevation in mutation frequency (p = 0.764) was seen for the lower dose rate (1.4 mGy/h, total dose 1.85 Gy; mutation frequency 13.74 ± 1.29 × 10−5 mutations/bp) compared with controls. This indicates that although the technique has the potential to detect an elevation in mutation frequency, it does not have sufficient sensitivity at the doses likely to be encountered in environmental monito
Type: Journal Article
URI: http://hdl.handle.net/1893/7472
DOI Link: http://dx.doi.org/10.1016/j.mrgentox.2005.10.011
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Westlakes Scientific Consulting Ltd
Westlakes Scientific Consulting Ltd
University of Liverpool
Biological and Environmental Sciences
Westlakes Scientific Consulting Ltd

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