Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7366

Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Chitinases from Vibrio: activity screening and purification of chiA from Vibrio carchariae
Authors: Suginta, Wipa
Robertson, Peter
Austin, Brian
Fry, Stephen C
Fothergill-Gilmore, Linda A
Contact Email: brian.austin@stir.ac.uk
Issue Date: Jul-2000
Publisher: Wiley-Blackwell
Citation: Suginta W, Robertson P, Austin B, Fry SC & Fothergill-Gilmore LA (2000) Chitinases from Vibrio: activity screening and purification of chiA from Vibrio carchariae, Journal of Applied Microbiology, 89 (1), pp. 76-84.
Abstract: Fourteen species of Vibrio were screened for chitin-induced chitinase activity in culture medium. V. carchariae, V. alginolyticus 283 and V. campbellii showed high levels of activity. Screening on agar plates containing swollen chitin showed high levels of chitinase activity by the same three species, and also by V. fischeri and V. alginolyticus 284. An affinity purification procedure was developed for the chitinase from V. carchariae. The purified chitinase was active as a monomer with Mr 63 000–66 000, and displayed activity toward polymeric chitin from acetylated chitosan or from crab shells. N-terminal sequence analysis and immunological cross-reactivity confirmed that the enzyme belongs to the group A/chiA family of bacterial chitinase
Type: Journal Article
URI: http://hdl.handle.net/1893/7366
DOI Link: http://dx.doi.org/10.1046/j.1365-2672.2000.01076.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Edinburgh
Heriot-Watt University
Heriot-Watt University
University of Edinburgh
University of Edinburgh

Files in This Item:
File Description SizeFormat 
austin_chitinasesfromvibrio_2000.pdf389.33 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.