Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7366
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dc.contributor.authorSuginta, Wipaen_UK
dc.contributor.authorRobertson, Peteren_UK
dc.contributor.authorAustin, Brianen_UK
dc.contributor.authorFry, Stephen Cen_UK
dc.contributor.authorFothergill-Gilmore, Linda Aen_UK
dc.date.accessioned2012-08-08T15:57:51Z-
dc.date.available2012-08-08T15:57:51Zen_UK
dc.date.issued2000-07en_UK
dc.identifier.urihttp://hdl.handle.net/1893/7366-
dc.description.abstractFourteen species of Vibrio were screened for chitin-induced chitinase activity in culture medium. V. carchariae, V. alginolyticus 283 and V. campbellii showed high levels of activity. Screening on agar plates containing swollen chitin showed high levels of chitinase activity by the same three species, and also by V. fischeri and V. alginolyticus 284. An affinity purification procedure was developed for the chitinase from V. carchariae. The purified chitinase was active as a monomer with Mr 63 000–66 000, and displayed activity toward polymeric chitin from acetylated chitosan or from crab shells. N-terminal sequence analysis and immunological cross-reactivity confirmed that the enzyme belongs to the group A/chiA family of bacterial chitinases.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationSuginta W, Robertson P, Austin B, Fry SC & Fothergill-Gilmore LA (2000) Chitinases from Vibrio: activity screening and purification of chiA from Vibrio carchariae. Journal of Applied Microbiology, 89 (1), pp. 76-84. https://doi.org/10.1046/j.1365-2672.2000.01076.xen_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.titleChitinases from Vibrio: activity screening and purification of chiA from Vibrio carchariaeen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate3000-01-01en_UK
dc.rights.embargoreason[austin_chitinasesfromvibrio_2000.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1046/j.1365-2672.2000.01076.xen_UK
dc.citation.jtitleJournal of Applied Microbiologyen_UK
dc.citation.issn1365-2672en_UK
dc.citation.issn1364-5072en_UK
dc.citation.volume89en_UK
dc.citation.issue1en_UK
dc.citation.spage76en_UK
dc.citation.epage84en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailbrian.austin@stir.ac.uken_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationHeriot-Watt Universityen_UK
dc.contributor.affiliationHeriot-Watt Universityen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.identifier.isiWOS:000088555100011en_UK
dc.identifier.wtid789341en_UK
dcterms.dateAccepted2000-07-31en_UK
dc.date.filedepositdate2012-08-08en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorSuginta, Wipa|en_UK
local.rioxx.authorRobertson, Peter|en_UK
local.rioxx.authorAustin, Brian|en_UK
local.rioxx.authorFry, Stephen C|en_UK
local.rioxx.authorFothergill-Gilmore, Linda A|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate3000-01-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameaustin_chitinasesfromvibrio_2000.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1364-5072en_UK
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