Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7223

Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Polymorphic microsatellite loci for the endangered pine hoverfly Blera fallax (Diptera: Syrphidae)
Authors: Rotheray, Ellen
Greminger, Maja P
Nater, Alexander
Krutzen, Michael
Goulson, Dave
Bussiere, Luc
Contact Email: dave.goulson@stir.ac.uk
Keywords: Syrphid
Pine hoverfly
454 pyrosequencing
Microsatellite
SSR
Conservation
Issue Date: Mar-2012
Publisher: Springer
Citation: Rotheray E, Greminger MP, Nater A, Krutzen M, Goulson D & Bussiere L (2012) Polymorphic microsatellite loci for the endangered pine hoverfly Blera fallax (Diptera: Syrphidae), Conservation Genetics Resources, 4 (1), pp. 117-120.
Abstract: We describe eleven polymorphic microsatellite loci developed for Blera fallax using ‘next generation’ 454 whole genome shotgun sequencing, along with conditions for three multiplex PCR reactions. We tested allelic variation on forty B. fallax individuals from Scotland and Sweden. Allelic richness and expected heterozygosity were 3.03 (±0.274) and 0.391 (±0.057) respectively. The number of alleles per locus ranged from 2 to 6. We anticipate that these loci will assist conservation management by allowing the monitoring of translocated populations, estimating effective population size, and assessing population structure and dispersal in Scotland and across Europe.
Type: Journal Article
URI: http://hdl.handle.net/1893/7223
DOI Link: http://dx.doi.org/10.1007/s12686-011-9488-2
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Biological and Environmental Sciences
University of Zurich
University of Zurich
University of Zurich
Biological and Environmental Sciences
Biological and Environmental Sciences

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