Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7170

Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Effect of parasitism on a nucleopolyhedrovirus amplified in Spodoptera frugiperda larvae parasitized by Campoletis sonorensis
Authors: Escribano, Ana
Williams, Trevor
Goulson, Dave
Cave, Ronald D
Chapman, Jason W
Caballero, Primitivo
Contact Email: dave.goulson@stir.ac.uk
Keywords: fall armyworm
baculovirus
serial passage
virulence
genotypic variants
per capita virus production
Issue Date: Dec-2000
Publisher: Wiley
Citation: Escribano A, Williams T, Goulson D, Cave RD, Chapman JW & Caballero P (2000) Effect of parasitism on a nucleopolyhedrovirus amplified in Spodoptera frugiperda larvae parasitized by Campoletis sonorensis, Entomologia Experimentalis et Applicata, 97 (3), pp. 257-264.
Abstract: We evaluated the consequences of parasitism by the solitary ichneumonid endoparasitoid Campoletis sonorensis (Cameron) towards the replication, genetic composition and virulence of a nucleopolyhedrovirus (Baculoviridae) originating from Spodoptera frugiperda (J. E. Smith) larvae. Parasitism by C. sonorensis and viral infection of third and fourth instar S. frugiperda larvae resulted in reduced growth compared with nonparasitized control larvae. A positive correlation was observed between virus yield and larval instar at the moment of infection. When larvae were virus-inoculated in the fourth instar, parasitism resulted in a significant reduction in mean per capita virus yield compared to the virus yield from nonparasitized larvae. In an experiment involving 10 serial passages of virus in both parasitized and nonparasitized larvae, restriction endonuclease analysis of viral DNA amplified in nonparasitized larvae revealed the presence of the wild-type virus as well as three additional variants (A, B, and C) diagnosed by the presence of novel submolar PstI fragments of different sizes. In contrast, analysis of viral DNA from parasitized larvae showed the presence of the wild-type virus and two other variants (E and F), each characterized by a different submolar BglII fragment. Southern blot analysis indicated that the submolar fragments of variants E and F contained sequences originating from the viral genome. Bioassay of the different virus variants in S. frugiperda larvae indicated that their virulence was equal or less than that of the wild-type virus. We conclude that parasitism can affect the quantity of virus produced in dually infected and parasitized larvae, but no adverse effects were detected in terms of the biological activity of the virus.
Type: Journal Article
URI: http://hdl.handle.net/1893/7170
DOI Link: http://dx.doi.org/10.1046/j.1570-7458.2000.00738.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Navarra
ECOSUR, Mexico
Biological and Environmental Sciences
Escuela Agrı́cola Panamericana, Hondur
University of Southampton
University of Navarra

Files in This Item:
File Description SizeFormat 
escribano_parasitism_2000.pdf102.75 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.