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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: A field-compatible method for measuring alternative respiratory pathway activities in vivo using stable O2 isotopes
Authors: Kornfeld, Ari
Horton, Travis W
Akir, Dan
Searle, Stephanie Y
Griffin, Kevin L
Atkin, Owen K
Subke, Jens-Arne
Turnbull, Matthew H
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Keywords: alternative oxidase
cytochrome c oxidase
oxygen isotope discrimination
stable isotope ratio mass spectrometry (IRMS)
Issue Date: Aug-2012
Publisher: Blackwell
Citation: Kornfeld A, Horton TW, Akir D, Searle SY, Griffin KL, Atkin OK, Subke J & Turnbull MH (2012) A field-compatible method for measuring alternative respiratory pathway activities in vivo using stable O2 isotopes, Plant, Cell and Environment, 35 (8), pp. 1518-1532.
Abstract: Plants can alter rates of electron transport through the alternative oxidase (AOX) pathway in response to environmental cues, thus modulating respiratory efficiency, but the 18O discrimination method necessary for measuring electron partitioning in vivo has been restricted to laboratory settings. To overcome this limitation, we developed a field-compatible analytical method. Series of plant tissue subsamples were incubated in 12 mL septum-capped vials for 0.5-4 h before aliquots of incubation air were injected into 3.7 mL evacuated storage vials. Vials were stored for up to 10 months before analysis by mass spectrometry. Measurements were corrected for unavoidable contamination. Additional mathematical tools were developed for detecting and addressing non-linearity (whether intrinsic or due to contamination) in the data used to estimate discrimination values. Initial contamination in the storage vials was 0.03 ± 0.01 atm; storing the gas samples at -17 °C eliminated further contamination effects over 10 months. Discrimination values obtained using our offline incubation and computation method replicated previously reported results over a range of 10-31‰, with precision generally better than ±0.5‰. Our method enables large-scale investigations of plant alternative respiration along natural environmental gradients under field conditions.
Type: Journal Article
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Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Canterbury
University of Canterbury
Weizmann Institute of Science
University of Canterbury
Columbia University
Australian National University
Biological and Environmental Sciences
University of Canterbury

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