Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2917
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.)
Authors: Jordal, Ann-Elise O
Torstensen, Bente E
Tsoi, Stephen
Tocher, Douglas R
Lall, Santosh P
Douglas, Susan E
Contact Email: drt1@stir.ac.uk
Keywords: Atlantic salmon
Salmo salar
fish ol
vegetable oil
gene expression
microarray
QPCR
Lipid metabolism
Issue Date: Oct-2005
Publisher: American Society for Nutritional Sciences
Citation: Jordal AO, Torstensen BE, Tsoi S, Tocher DR, Lall SP & Douglas SE (2005) Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.), Journal of Nutrition, 135 (10), pp. 2355-2361.
Abstract: Supplies of marine fish oils (FO ) are limited, and sustainable production in aquaculture dictates that alternatives, such as vegetable oils, must be found that do not compromise fish health and product quality. Nutrigenomics is anticipated to provide an increased understanding of how nutrition influences metabolic pathways and homeostatic control, and may be used to measure and validate subtle changes in organ-specific, metabolic gene expression signatures. We have compared two groups of Atlantic salmon fed diets containing 100% FO or 75% rapeseed oil (RO)/25% FO for 42 weeks. A small-scale cDNA microarray was constructed to screen for changes in expression of lipid metabolism genes in the liver resulting from this partial substitution of RO for FO. Fatty acid desaturase gene expression was significantly increased in fish fed 75% RO compared to fish fed the control diet; this was confirmed by quantitative Real Time PCR analysis (Q-PCR). In addition, several genes, among these mitochondrial proteins, peroxisome proliferator activated receptor-γ (PPARγ) as well as other transcription factors, co-activators and signal transducers, showed significant differential regulation. This partially validated microarray may be used for further gene expression profiling using other dietary comparison
Type: Journal Article
URI: http://hdl.handle.net/1893/2917
URL: http://jn.nutrition.org/content/135/10/2355.abstract
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.
Affiliation: National Institute of Nutrition and Seafood Research (NIFES)
National Institute of Nutrition and Seafood Research (NIFES)
National Institute of Nutrition and Seafood Research (NIFES)
Aquaculture
National Institute of Nutrition and Seafood Research (NIFES)
National Institute of Nutrition and Seafood Research (NIFES)

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