Please use this identifier to cite or link to this item:
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: How do enzymes catalysing soil nitrogen transformations respond to changing temperatures?
Authors: Fraser, Fiona
Hallett, Paul D.
Wookey, Philip
Hartley, Iain
Hopkins, David
Contact Email:
Keywords: Nitrogen mineralisation
Issue Date: Jan-2013
Citation: Fraser F, Hallett PD, Wookey P, Hartley I & Hopkins D (2013) How do enzymes catalysing soil nitrogen transformations respond to changing temperatures?, Biology and Fertility of Soils, 49 (1), pp. 99-103.
Abstract: Biological processes in soils are regulated in part by soil temperature, and there is currently considerable interest in obtaining robust information on the temperature sensitivity of carbon cycling process. However, very little comparable information exists on the temperature regulation of specific nitrogen cycling processes. This paper addresses this problem by measuring the temperature sensitivity of nitrogen cycling enzymes in soil. A grassland soil was incubated over a range of temperatures (-2 to 21 °C) reflecting 99 % of the soil temperature range during the previous 50 years at the site. After 7 and 14 days of incubation, potential activities of protease, amidase and urease were determined. Activities of protease and urease were positively related to temperature (activation energy; Ea = 49. 7 and 73. 4 kJ mol-1, respectively, and Q10 = 2. 97 and 2. 78, respectively). By contrast, amidase activity was relatively insensitive to temperature, but the activity was significantly increased after the addition of glucose. This indicated that there was a stoichiometric imbalance with amidase activity only being triggered when there was a supply of exogenous carbon. Thus, carbon supply was a greater constraint to amidase activity than temperature was in this particular soil. © 2012 Springer-Verlag.
DOI Link:
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.

Files in This Item:
File Description SizeFormat 
Fraser_etal_BFS_2013.pdf175.8 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.