Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2567
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper
Authors: Minghetti, Matteo
Leaver, Michael
Carpene, Emilio
George, Stephen
Contact Email: matteo.minghetti@stir.ac.uk
Keywords: copper
Sparus aurata
dietary copper
waterborne copper
Ctr1
gene expression
metallothionein
glutathione reductase
Issue Date: May-2008
Publisher: Elsevier
Citation: Minghetti M, Leaver M, Carpene E & George S (2008) Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper, Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 147 (4), pp. 450-459.
Abstract: The high affinity copper transporter 1 (Ctr1), metallothionein (MT) and glutathione reductase (GR) are essential for copper uptake, sequestration and defense respectively. Following rearing on a normal commercial diet (12.6±0.2 mgKg-1Cu), sea bream were fed an experimental control diet lacking mineral mix (7.7±0.3 mgKg-1Cu), an experimental diet enhanced with Cu (135±4 mgKg-1 Cu) or an experimental diet (7.7±0.3 mgKg-1Cu) while exposed to Cu in water (0.294±0.013mgL-1). Fish were sampled at 0, 15 and 30 d after exposures. Fish fed the Cu-enhanced experimental diet showed lower levels of expression of Ctr1 in the intestine and liver compared to fish fed control experimental diets, whilst Ctr1 expression in the gill and kidney was unaffected by excess dietary Cu exposure. Waterborne Cu-exposure increased Ctr1 mRNA levels in the intestine and the kidney compared to experimental controls. Excess dietary Cu exposure had no effect on levels of metallothionein (MT) mRNA, and the only effect of dietary excess Cu on glutathione reductase (GR) mRNA was a decrease in the intestine. Both MT mRNA and GR were increased in the liver and gill after waterborne Cu exposure, compared to levels in fish fed experimental control low Cu diets. Thus, Ctr1, MT and GR mRNA expression in response to excess Cu is dependent on the route of exposure. Furthermore, the tissue expression profile of sea bream Ctr1 is consistent with the known physiology of copper exposure in fish and indicates a role both in essential copper uptake and in avoidance of excess dietary and waterborne copper in
Type: Journal Article
URI: http://hdl.handle.net/1893/2567
URL: http://www.sciencedirect.com/science/journal/15320456
DOI Link: http://dx.doi.org/10.1016/j.cbpc.2008.01.014
Rights: Published in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology by Elsevier.
Affiliation: Aquaculture
Aquaculture
University of Bologna
Aquaculture

Files in This Item:
File Description SizeFormat 
Minghetti15323complete.pdf582.21 kBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.