Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2567
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMinghetti, Matteoen_UK
dc.contributor.authorLeaver, Michaelen_UK
dc.contributor.authorCarpene, Emilioen_UK
dc.contributor.authorGeorge, Stephenen_UK
dc.date.accessioned2012-09-03T12:10:12Z-
dc.date.available2012-09-03T12:10:12Z-
dc.date.issued2008-05en_UK
dc.identifier.urihttp://hdl.handle.net/1893/2567-
dc.description.abstractThe high affinity copper transporter 1 (Ctr1), metallothionein (MT) and glutathione reductase (GR) are essential for copper uptake, sequestration and defense respectively. Following rearing on a normal commercial diet (12.6±0.2 mgKg-1Cu), sea bream were fed an experimental control diet lacking mineral mix (7.7±0.3 mgKg-1Cu), an experimental diet enhanced with Cu (135±4 mgKg-1 Cu) or an experimental diet (7.7±0.3 mgKg-1Cu) while exposed to Cu in water (0.294±0.013mgL-1). Fish were sampled at 0, 15 and 30 d after exposures. Fish fed the Cu-enhanced experimental diet showed lower levels of expression of Ctr1 in the intestine and liver compared to fish fed control experimental diets, whilst Ctr1 expression in the gill and kidney was unaffected by excess dietary Cu exposure. Waterborne Cu-exposure increased Ctr1 mRNA levels in the intestine and the kidney compared to experimental controls. Excess dietary Cu exposure had no effect on levels of metallothionein (MT) mRNA, and the only effect of dietary excess Cu on glutathione reductase (GR) mRNA was a decrease in the intestine. Both MT mRNA and GR were increased in the liver and gill after waterborne Cu exposure, compared to levels in fish fed experimental control low Cu diets. Thus, Ctr1, MT and GR mRNA expression in response to excess Cu is dependent on the route of exposure. Furthermore, the tissue expression profile of sea bream Ctr1 is consistent with the known physiology of copper exposure in fish and indicates a role both in essential copper uptake and in avoidance of excess dietary and waterborne copper influx.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMinghetti M, Leaver M, Carpene E & George S (2008) Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 147 (4), pp. 450-459. http://www.sciencedirect.com/science/journal/15320456; https://doi.org/10.1016/j.cbpc.2008.01.014en_UK
dc.rightsPublished in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology by Elsevier.en_UK
dc.subjectcopperen_UK
dc.subjectSparus aurataen_UK
dc.subjectdietary copperen_UK
dc.subjectwaterborne copperen_UK
dc.subjectCtr1en_UK
dc.subjectgene expressionen_UK
dc.subjectmetallothioneinen_UK
dc.subjectglutathione reductaseen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectDietary supplementsen_UK
dc.subjectLipoproteins Fishen_UK
dc.titleCopper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copperen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.cbpc.2008.01.014en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part C: Toxicology and Pharmacologyen_UK
dc.citation.issn1532-0456en_UK
dc.citation.volume147en_UK
dc.citation.issue4en_UK
dc.citation.spage450en_UK
dc.citation.epage459en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.identifier.urlhttp://www.sciencedirect.com/science/journal/15320456en_UK
dc.author.emailmatteo.minghetti@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Bolognaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000255506400007en_UK
dc.identifier.scopusid2-s2.0-41449085129en_UK
dc.identifier.wtid837576en_UK
dc.contributor.orcid0000-0002-3155-0844en_UK
dcterms.dateAccepted2008-05-31en_UK
dc.date.filedepositdate2010-11-22en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMinghetti, Matteo|en_UK
local.rioxx.authorLeaver, Michael|0000-0002-3155-0844en_UK
local.rioxx.authorCarpene, Emilio|en_UK
local.rioxx.authorGeorge, Stephen|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2010-11-22en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2010-11-22|en_UK
local.rioxx.filenameMinghetti15323complete.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1532-0456en_UK
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
Minghetti15323complete.pdfFulltext - Accepted Version582.21 kBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.