Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25086
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Optimizing electron backscatter diffraction of carbonate biominerals - resin type and carbon coating
Authors: Pérez-Huerta, Alberto
Cusack, Maggie
Contact Email: maggie.cusack@stir.ac.uk
Keywords: aragonite
calcite
mussel
epoxy
diffraction intensity
nanometer
Issue Date: Jun-2009
Citation: Pérez-Huerta A & Cusack M (2009) Optimizing electron backscatter diffraction of carbonate biominerals - resin type and carbon coating, Microscopy and Microanalysis, 15 (3), pp. 197-203.
Abstract: Electron backscatter diffraction (EBSD) is becoming a widely used technique to determine crystallographic orientation in biogenic carbonates. Despite this use, there is little information available on preparation for the analysis of biogenic carbonates. EBSD data are compared for biogenic aragonite and calcite in the common blue mussel, Mytilus edulis, using different types of resin and thicknesses of carbon coating. Results indicate that carbonate biomineral samples provide better EBSD results if they are embedded in resin, particularly epoxy resin. A uniform layer of carbon of 2.5 nm thickness provides sufficient conductivity for EBSD analyses of such insulators to avoid charging without masking the diffracted signal. Diffraction intensity decreases with carbon coating thickness of 5 nm or more. This study demonstrates the importance of optimizing sample preparation for EBSD analyses of insulators such as carbonate biominerals. © 2009 Microscopy Society of America.
DOI Link: http://dx.doi.org/10.1017/S1431927609090370
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