Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24991
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Oscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies
Authors: Subramanian, Mahendran
Tyler, Aimee-Jayne
Luther, Eva Maria
Di, Daniel Elena
Lim, Jenson
Dobson, John
Contact Email: jenson.lim@stir.ac.uk
Keywords: magnetic nanoparticles
transfection
SH-SY5Y
hippocampal neurons
cortical neurons
non-viral gene delivery
nanomagnetic gene transfection
biomagnetics
gradient magnetic field
permanent magnets
Issue Date: 29-Jan-2017
Citation: Subramanian M, Tyler A, Luther EM, Di Daniel E, Lim J & Dobson J (2017) Oscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies, Nanomaterials, 7 (2), Art. No.: 28.
Abstract: To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types.
URL: http://www.mdpi.com/2079-4991/7/2/28
DOI Link: http://dx.doi.org/10.3390/nano7020028
Rights: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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