|Appears in Collections:||Biological and Environmental Sciences Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Purification and characterization of thaumatopain, a cysteine protease from the arils of the plant Thaumatococcus daniellii|
Stephen, Andrew G
|Citation:||Cusack M, Stephen AG, Powls R & Beynon R (1991) Purification and characterization of thaumatopain, a cysteine protease from the arils of the plant Thaumatococcus daniellii, Biochemical Journal, 274 (1), pp. 231-236.|
|Abstract:||Aqueous extracts of the aril of the seed of Thaumatococcus daniellii contain, in addition to the intensely sweet protein thaumatin, a cysteine protease that we have termed thaumatopain. Thaumatopain has been purified by ion-exchange chromatography from arils, and is a monomeric protein of M(r) 30000. The protease strongly resembles papain in proteolytic activity, pH optima, susceptibility to inhibitors of cysteine proteases and in N-terminal sequence. The protease has also been identified in crude aril extracts by affinity labelling with iodo[14C]acetate. Thaumatopain is responsible for the cysteine protease activity previously attributed to thaumatin. Thaumatin is digested by thaumatopain at neutral to alkaline pH values.|
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