Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24770
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Deregulated expression of HDAC9 in B cells promotes development of lymphoproliferative disease and lymphoma in mice
Authors: Gil, Veronica
Bhagat, Govind
Howell, Louise
Zhang, Jiyuan
Kim, Chae H
Stengel, Sven
Vega, Francisco
Zelent, Arthur
Petrie, Kevin
Contact Email: kevin.petrie@stir.ac.uk
Keywords: HDAC9
Lymphoma
Transgenic mouse
Issue Date: 2016
Citation: Gil V, Bhagat G, Howell L, Zhang J, Kim CH, Stengel S, Vega F, Zelent A & Petrie K (2016) Deregulated expression of HDAC9 in B cells promotes development of lymphoproliferative disease and lymphoma in mice, Disease Models and Mechanisms, 9 (12), pp. 1483-1495.
Abstract: Histone deacetylase 9 (HDAC9) is expressed in B cells, and its overexpression has been observed in B-lymphoproliferative disorders, including B-cell non-Hodgkin lymphoma (B-NHL). We examined HDAC9 protein expression and copy number alterations in primary B-NHL samples, identifying high HDAC9 expression among various lymphoma entities andHDAC9copy number gains in 50% of diffuse large B-cell lymphoma (DLBCL). To study the role of HDAC9 in lymphomagenesis, we generated a genetically engineered mouse (GEM) model that constitutively expressed anHDAC9transgene throughout B-cell development under the control of the immunoglobulin heavy chain (IgH) enhancer (Eμ). Here, we report that theEμ-HDAC9GEM model develops splenic marginal zone lymphoma and lymphoproliferative disease (LPD) with progression towards aggressive DLBCL, with gene expression profiling supporting a germinal center cell origin, as is also seen in human B-NHL tumors. Analysis ofEμ-HDAC9tumors suggested that HDAC9 might contribute to lymphomagenesis by altering pathways involved in growth and survival, as well as modulating BCL6 activity and p53 tumor suppressor function. Epigenetic modifications play an important role in the germinal center response, and deregulation of the B-cell epigenome as a consequence of mutations and other genomic aberrations are being increasingly recognized as important steps in the pathogenesis of a variety of B-cell lymphomas. A thorough mechanistic understanding of these alterations will inform the use of targeted therapies for these malignancies. These findings strongly suggest a role for HDAC9 in B-NHL and establish a novel GEM model for the study of lymphomagenesis and, potentially, preclinical testing of therapeutic approaches based on histone deacetylase inhibitors.
DOI Link: http://dx.doi.org/10.1242/dmm.023366
Rights: © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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