Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24731
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Proteome analysis of the UVB-resistant marine bacterium Photobacterium angustum S14
Authors: Matallana-Surget, Sabine
Joux, Fabien
Wattiez, Ruddy
Lebaron, Philippe
Contact Email: sabine.matallanasurget@stir.ac.uk
Issue Date: 1-Aug-2012
Citation: Matallana-Surget S, Joux F, Wattiez R & Lebaron P (2012) Proteome analysis of the UVB-resistant marine bacterium Photobacterium angustum S14, PLoS ONE, 7 (8), Art. No.: e42299.
Abstract: The proteome of the marine bacterium Photobacterium angustum S14 was exposed to UVB and analyzed by the implementation of both the post-digest ICPL labeling method and 2D-DIGE technique using exponentially growing cells. A total of 40 and 23 proteins were quantified in all replicates using either the ICPL or 2D-DIGE methods, respectively. By combining both datasets from 8 biological replicates (4 biological replicates for each proteomics technique), 55 proteins were found to respond significantly to UVB radiation in P. angustum. A total of 8 UVB biomarkers of P. angustum were quantified in all replicates using both methods. Among them, the protein found to present the highest increase in abundance (almost a 3-fold change) was RecA, which is known to play a crucial role in the so-called recombinational repair process. We also observed a high number of antioxidants, transport proteins, metabolism-related proteins, transcription/translation regulators, chaperonins and proteases. We also discuss and compare the UVB response and global protein expression profiles obtained for two different marine bacteria with trophic lifestyles: the copiotroph P. angustum and oligotroph Sphingopyxis alaskensis.
DOI Link: http://dx.doi.org/10.1371/journal.pone.0042299
Rights: © Matallana-Surget et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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