Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24109
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Gene expansion shapes genome architecture in the human pathogen Lichtheimia corymbifera: an evolutionary genomics analysis in the ancient terrestrial mucorales (Mucoromycotina)
Authors: Schwartze, Volker U
Winter, Sascha
Shelest, Ekaterina
Marcet-Houben, Marina
Horn, Fabian
Wehner, Stefanie
Linde, Jorg
Valiante, Vito
Sammeth, Michael
Riege, Konstantin
Nowrousian, Minou
Kaerger, Kerstin
Jacobsen, Ilse D
Marz, Manja
Brakhage, Axel A
Contact Email: stefanie.wehner@stir.ac.uk
Issue Date: 14-Aug-2014
Publisher: Public Library of Science
Citation: Schwartze VU, Winter S, Shelest E, Marcet-Houben M, Horn F, Wehner S, Linde J, Valiante V, Sammeth M, Riege K, Nowrousian M, Kaerger K, Jacobsen ID, Marz M & Brakhage AA (2014) Gene expansion shapes genome architecture in the human pathogen Lichtheimia corymbifera: an evolutionary genomics analysis in the ancient terrestrial mucorales (Mucoromycotina), PLoS Genetics, 10 (8), Art. No.: e1004496.
Abstract: Lichtheimia species are the second most important cause of mucormycosis in Europe. To provide broader insights into the molecular basis of the pathogenicity-associated traits of the basal Mucorales, we report the full genome sequence of L. corymbifera and compared it to the genome of Rhizopus oryzae, the most common cause of mucormycosis worldwide. The genome assembly encompasses 33.6 MB and 12,379 protein-coding genes. This study reveals four major differences of the L. corymbifera genome to R. oryzae: (i) the presence of an highly elevated number of gene duplications which are unlike R. oryzae not due to whole genome duplication (WGD), (ii) despite the relatively high incidence of introns, alternative splicing (AS) is not frequently observed for the generation of paralogs and in response to stress, (iii) the content of repetitive elements is strikingly low (<5%), (iv) L. corymbifera is typically haploid. Novel virulence factors were identified which may be involved in the regulation of the adaptation to iron-limitation, e.g. LCor01340.1 encoding a putative siderophore transporter and LCor00410.1 involved in the siderophore metabolism. Genes encoding the transcription factors LCor08192.1 and LCor01236.1, which are similar to GATA type regulators and to calcineurin regulated CRZ1, respectively, indicating an involvement of the calcineurin pathway in the adaption to iron limitation. Genes encoding MADS-box transcription factors are elevated up to 11 copies compared to the 1–4 copies usually found in other fungi. More findings are: (i) lower content of tRNAs, but unique codons inL. corymbifera, (ii) Over 25% of the proteins are apparently specific for L. corymbifera. (iii) L. corymbifera contains only 2/3 of the proteases (known to be essential virulence factors) in comparision to R. oryzae. On the other hand, the number of secreted proteases, however, is roughly twice as high as in R. oryzae.
Type: Journal Article
URI: http://hdl.handle.net/1893/24109
DOI Link: http://dx.doi.org/10.1371/journal.pgen.1004496
Rights: © 2014 Schwartze et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Affiliation: Friedrich Schiller University of Jena
Friedrich Schiller University of Jena
Leibniz Institute for Natural Product Research and Infection Biology
Universitat Pompeu Fabra
Leibniz Institute for Natural Product Research and Infection Biology
Aquaculture
Leibniz Institute for Natural Product Research and Infection Biology
Leibniz Institute for Natural Product Research and Infection Biology
Centre Nacional d'Anàlisi Genòmica (CNAG), Spain
Friedrich Schiller University of Jena
Ruhr-University of Bochum, Germany
Friedrich Schiller University of Jena
Friedrich Schiller University of Jena
Philipps University of Marburg
Friedrich Schiller University of Jena

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