Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24033
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Proteome-wide analysis and diel proteomic profiling in the cyanobacterium Arthrospira platensis PCC 8005
Authors: Matallana, Surget Sabine
Derock, Jérémy
Baptiste, Leroy
Badri, Hanene
Deschoenmaeker, Frederic
Wattiez, Ruddy
Contact Email: sabine.matallanasurget@stir.ac.uk
Issue Date: 10-Jun-2014
Publisher: Public Library of Science
Citation: Matallana Surget S, Derock J, Baptiste L, Badri H, Deschoenmaeker F & Wattiez R (2014) Proteome-wide analysis and diel proteomic profiling in the cyanobacterium Arthrospira platensis PCC 8005, PLoS ONE, 9 (6), Art. No.: e99076.
Abstract: The filamentous cyanobacteriumArthrospira platensishas a long history of use as a food supply and it has been used by the European Space Agency in the MELiSSA project, an artificial microecosystem which supports life during long-term manned space missions. This study assesses progress in the field of cyanobacterial shotgun proteomics and light/dark diurnal cycles by focusing onArthrospira platensis. Several fractionation workflows including gel-free and gel-based protein/peptide fractionation procedures were used and combined with LC-MS/MS analysis, enabling the overall identification of 1306 proteins, which represents 21% coverage of the theoretical proteome. A total of 30 proteins were found to be significantly differentially regulated under light/dark growth transition. Interestingly, most of the proteins showing differential abundance were related to photosynthesis, the Calvin cycle and translation processes. A novel aspect and major achievement of this work is the successful improvement of the cyanobacterial proteome coverage using a 3D LC-MS/MS approach, based on an immobilized metal affinity chromatography, a suitable tool that enabled us to eliminate the most abundant protein, the allophycocyanin. We also demonstrated that cell growth follows a light/dark cycle inA. platensis. This preliminary proteomic study has highlighted new characteristics of theArthrospira platensisproteome in terms of diurnal regulation.
Type: Journal Article
URI: http://hdl.handle.net/1893/24033
DOI Link: http://dx.doi.org/10.1371/journal.pone.0099076
Rights: © 2014 Matallana-Surget et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Affiliation: Biological and Environmental Sciences
University of Mons
University of Mons
University of Mons
University of Mons
University of Mons

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