Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22808
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015
Authors: Faye, Oumar
Faye, Ousmane
Soropogui, Barre
Patel, Pranav
El, Wahed Ahmed Abd
Loucoubar, Cheikh
Fall, Gamou
Kiory, Davy
Magassouba, N'Faly
Keita, Sakoba
Konde, Mandy Kader
Diallo, Alpha Amadou
Koivogui, Lamine
Karlberg, Helen
Weidmann, Manfred
Contact Email: m.w.weidmann@stir.ac.uk
Issue Date: 5-Nov-2015
Publisher: European Centre for Disease Prevention and Control
Citation: Faye O, Faye O, Soropogui B, Patel P, El Wahed AA, Loucoubar C, Fall G, Kiory D, Magassouba N, Keita S, Konde MK, Diallo AA, Koivogui L, Karlberg H & Weidmann M (2015) Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015, Eurosurveillance, 20 (44), Art. No.: 30053.
Abstract: In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription recombinase polymerase amplification assay for the detection of Ebola virus (EBOV-RT-RPA). The performance of combined extraction and detection was best for swabs. Sensitivity and specificity of the combined SE and EBOV-RT-RPA were tested in a mobile laboratory consisting of a mobile glovebox and a Diagnostics-in-a-Suitcase powered by a battery and solar panel, deployed to Matoto Conakry, Guinea as part of the reinforced surveillance strategy in April 2015 to reach the goal of zero cases. The EBOV-RT-RPA was evaluated in comparison to two real-time PCR assays. Of 928 post-mortem swabs, 120 tested positive, and the combined SE and EBOV-RT-RPA yielded a sensitivity and specificity of 100% in reference to one real-time RT-PCR assay. Another widely used real-time RT-PCR was much less sensitive than expected. Results were provided very fast within 30 to 60 min, and the field deployment of the mobile laboratory helped improve burial management and community engagement.
Type: Journal Article
URI: http://hdl.handle.net/1893/22808
URL: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=21289
DOI Link: http://dx.doi.org/10.2807/1560-7917.ES.2015.20.44.30053
Rights: Published under the Creative Commons Attribution (CC BY) licence. You are free to share and adapt the material, but you must give appropriate credit, provide a link to the licence, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Notes: Additional co-authors: Ali Mirazimi, Oliver Nentwich, Olaf Piepenburg, Matthias Niedrig, Amadou Alpha Sall
Affiliation: Institut Pasteur de Dakar
Institut Pasteur de Dakar
Laboratory for Hemorrhagic Fevers of Guinea
Robert Koch Institute
German Primate Center
Institut Pasteur de Dakar
Institut Pasteur de Dakar
Institut Pasteur de Dakar
Laboratory for Hemorrhagic Fevers of Guinea
Ministry of Health & Public Hygiene (Guinea)
Center Of Excellence For Training, Research On Malaria & Priority Diseases In Guinea (CEFORPAG)
Ministry of Health & Public Hygiene (Guinea)
Gamal Abdel Nasser University of Conakry
Karolinska Institutet
Aquaculture

Files in This Item:
File Description SizeFormat 
Faye et al_Eurosurveillance_2015.pdf982.78 kBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.