Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22808
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dc.contributor.authorFaye, Oumaren_UK
dc.contributor.authorFaye, Ousmaneen_UK
dc.contributor.authorSoropogui, Barreen_UK
dc.contributor.authorPatel, Pranaven_UK
dc.contributor.authorEl Wahed, Ahmed Abden_UK
dc.contributor.authorLoucoubar, Cheikhen_UK
dc.contributor.authorFall, Gamouen_UK
dc.contributor.authorKiory, Davyen_UK
dc.contributor.authorMagassouba, N'Falyen_UK
dc.contributor.authorKeita, Sakobaen_UK
dc.contributor.authorKonde, Mandy Kaderen_UK
dc.contributor.authorDiallo, Alpha Amadouen_UK
dc.contributor.authorKoivogui, Lamineen_UK
dc.contributor.authorKarlberg, Helenen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.date.accessioned2016-05-06T00:36:27Z-
dc.date.available2016-05-06T00:36:27Z-
dc.date.issued2015-11-05en_UK
dc.identifier.other30053en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22808-
dc.description.abstractIn the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription recombinase polymerase amplification assay for the detection of Ebola virus (EBOV-RT-RPA). The performance of combined extraction and detection was best for swabs. Sensitivity and specificity of the combined SE and EBOV-RT-RPA were tested in a mobile laboratory consisting of a mobile glovebox and a Diagnostics-in-a-Suitcase powered by a battery and solar panel, deployed to Matoto Conakry, Guinea as part of the reinforced surveillance strategy in April 2015 to reach the goal of zero cases. The EBOV-RT-RPA was evaluated in comparison to two real-time PCR assays. Of 928 post-mortem swabs, 120 tested positive, and the combined SE and EBOV-RT-RPA yielded a sensitivity and specificity of 100% in reference to one real-time RT-PCR assay. Another widely used real-time RT-PCR was much less sensitive than expected. Results were provided very fast within 30 to 60 min, and the field deployment of the mobile laboratory helped improve burial management and community engagement.en_UK
dc.language.isoenen_UK
dc.publisherEuropean Centre for Disease Prevention and Controlen_UK
dc.relationFaye O, Faye O, Soropogui B, Patel P, El Wahed AA, Loucoubar C, Fall G, Kiory D, Magassouba N, Keita S, Konde MK, Diallo AA, Koivogui L, Karlberg H & Weidmann M (2015) Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015. Eurosurveillance, 20 (44), Art. No.: 30053. http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=21289; https://doi.org/10.2807/1560-7917.ES.2015.20.44.30053en_UK
dc.rightsPublished under the Creative Commons Attribution (CC BY) licence. You are free to share and adapt the material, but you must give appropriate credit, provide a link to the licence, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.titleDevelopment and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015en_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.2807/1560-7917.ES.2015.20.44.30053en_UK
dc.identifier.pmid26558690en_UK
dc.citation.jtitleEurosurveillanceen_UK
dc.citation.issn1025-496Xen_UK
dc.citation.issn1560-7917en_UK
dc.citation.volume20en_UK
dc.citation.issue44en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.identifier.urlhttp://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=21289en_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.citation.date05/11/2015en_UK
dc.description.notesAdditional co-authors: Ali Mirazimi, Oliver Nentwich, Olaf Piepenburg, Matthias Niedrig, Amadou Alpha Sallen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationLaboratory for Hemorrhagic Fevers of Guineaen_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationGerman Primate Centeren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationLaboratory for Hemorrhagic Fevers of Guineaen_UK
dc.contributor.affiliationMinistry of Health & Public Hygiene (Guinea)en_UK
dc.contributor.affiliationCenter Of Excellence For Training, Research On Malaria & Priority Diseases In Guinea (CEFORPAG)en_UK
dc.contributor.affiliationMinistry of Health & Public Hygiene (Guinea)en_UK
dc.contributor.affiliationGamal Abdel Nasser University of Conakryen_UK
dc.contributor.affiliationKarolinska Instituteten_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000364495000002en_UK
dc.identifier.scopusid2-s2.0-84947092955en_UK
dc.identifier.wtid584480en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2015-09-28en_UK
dcterms.dateAccepted2015-09-28en_UK
dc.date.filedepositdate2016-01-28en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorFaye, Oumar|en_UK
local.rioxx.authorFaye, Ousmane|en_UK
local.rioxx.authorSoropogui, Barre|en_UK
local.rioxx.authorPatel, Pranav|en_UK
local.rioxx.authorEl Wahed, Ahmed Abd|en_UK
local.rioxx.authorLoucoubar, Cheikh|en_UK
local.rioxx.authorFall, Gamou|en_UK
local.rioxx.authorKiory, Davy|en_UK
local.rioxx.authorMagassouba, N'Faly|en_UK
local.rioxx.authorKeita, Sakoba|en_UK
local.rioxx.authorKonde, Mandy Kader|en_UK
local.rioxx.authorDiallo, Alpha Amadou|en_UK
local.rioxx.authorKoivogui, Lamine|en_UK
local.rioxx.authorKarlberg, Helen|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2016-01-28en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2016-01-28|en_UK
local.rioxx.filenameFaye et al_Eurosurveillance_2015.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1560-7917en_UK
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