Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22781
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Examination of the early infection stages of koi herpesvirus (KHV) in experimentally infected carp, Cyprinus carpio L. using in situ hybridisation
Other Titles: Early stages of KHV using ISH
Authors: Monaghan, Sean
Thompson, Kimberly
Adams, Alexandra
Kempter, Jolanta
Bergmann, Sven
Contact Email: s.j.monaghan@stir.ac.uk
Keywords: cyprinid herpesvirus 3
early infection
in situ hybridization
koi herpesvirus
pathogenesis
Issue Date: May-2015
Publisher: Wiley-Blackwell
Citation: Monaghan S, Thompson K, Adams A, Kempter J & Bergmann S (2015) Examination of the early infection stages of koi herpesvirus (KHV) in experimentally infected carp, Cyprinus carpio L. using in situ hybridisation, Journal of Fish Diseases, 38 (5), pp. 477-489.
Abstract: Koi herpesvirus (KHV) causes a highly infectious disease afflicting common carp and koi,Cyprinus carpioL. Various molecular and antibody-based detection methods have been used to elucidate the rapid attachment and dissemination of the virus throughout carp tissues, facilitating ongoing development of effective diagnostic approaches.In situhybridization (ISH) was used here to determine the target tissues of KHV during very early infection, after infecting carp with a highly virulent KHV isolate. Analysis of paraffin-embedded tissues (i.e. gills, skin, spleen, kidney, gut, liver and brain) during the first 8h and following 10days post-infection (hpi; dpi) revealed positive signals in skin mucus, gills and gut sections after only 1hpi. Respiratory epithelial cells were positive as early as 2hpi. Viral DNA was also detected within blood vessels of various tissues early in the infection. Notable increases in signal abundance were observed in the gills and kidney between 5 and 10 dpi, and viral DNA was detected in all tissues except brain. This study suggests that the gills and gut play an important role in the early pathogenesis of thisAlloherpesvirus,in addition to skin, and demonstrates ISH as a useful diagnostic tool for confirmation of acutely infected carp.
Type: Journal Article
URI: http://hdl.handle.net/1893/22781
DOI Link: http://dx.doi.org/10.1111/jfd.12260
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Aquaculture
Aquaculture
West Pomeranian University of Technology
Friedrich-Loeffler Institut

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