Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22781
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dc.contributor.authorMonaghan, Seanen_UK
dc.contributor.authorThompson, Kimberlyen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.contributor.authorKempter, Jolantaen_UK
dc.contributor.authorBergmann, Svenen_UK
dc.date.accessioned2016-01-27T00:26:07Z-
dc.date.available2016-01-27T00:26:07Z-
dc.date.issued2015-05en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22781-
dc.description.abstractKoi herpesvirus (KHV) causes a highly infectious disease afflicting common carp and koi,Cyprinus carpioL. Various molecular and antibody-based detection methods have been used to elucidate the rapid attachment and dissemination of the virus throughout carp tissues, facilitating ongoing development of effective diagnostic approaches.In situhybridization (ISH) was used here to determine the target tissues of KHV during very early infection, after infecting carp with a highly virulent KHV isolate. Analysis of paraffin-embedded tissues (i.e. gills, skin, spleen, kidney, gut, liver and brain) during the first 8h and following 10days post-infection (hpi; dpi) revealed positive signals in skin mucus, gills and gut sections after only 1hpi. Respiratory epithelial cells were positive as early as 2hpi. Viral DNA was also detected within blood vessels of various tissues early in the infection. Notable increases in signal abundance were observed in the gills and kidney between 5 and 10 dpi, and viral DNA was detected in all tissues except brain. This study suggests that the gills and gut play an important role in the early pathogenesis of thisAlloherpesvirus,in addition to skin, and demonstrates ISH as a useful diagnostic tool for confirmation of acutely infected carp.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationMonaghan S, Thompson K, Adams A, Kempter J & Bergmann S (2015) Examination of the early infection stages of koi herpesvirus (KHV) in experimentally infected carp, Cyprinus carpio L. using in situ hybridisation [Early stages of KHV using ISH]. Journal of Fish Diseases, 38 (5), pp. 477-489. https://doi.org/10.1111/jfd.12260en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectcyprinid herpesvirus 3en_UK
dc.subjectearly infectionen_UK
dc.subjectin situ hybridizationen_UK
dc.subjectkoi herpesvirusen_UK
dc.subjectpathogenesisen_UK
dc.titleExamination of the early infection stages of koi herpesvirus (KHV) in experimentally infected carp, Cyprinus carpio L. using in situ hybridisationen_UK
dc.title.alternativeEarly stages of KHV using ISHen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-14en_UK
dc.rights.embargoreason[Monaghan_et_al-2015-Journal_of_Fish_Diseases.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1111/jfd.12260en_UK
dc.identifier.pmid24925228en_UK
dc.citation.jtitleJournal of Fish Diseasesen_UK
dc.citation.issn1365-2761en_UK
dc.citation.issn0140-7775en_UK
dc.citation.volume38en_UK
dc.citation.issue5en_UK
dc.citation.spage477en_UK
dc.citation.epage489en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emails.j.monaghan@stir.ac.uken_UK
dc.citation.date13/06/2014en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationWest Pomeranian University of Technologyen_UK
dc.contributor.affiliationFriedrich-Loeffler Instituten_UK
dc.identifier.isiWOS:000352582600006en_UK
dc.identifier.scopusid2-s2.0-84926416234en_UK
dc.identifier.wtid588283en_UK
dc.contributor.orcid0000-0002-7692-7756en_UK
dc.date.accepted2014-03-20en_UK
dcterms.dateAccepted2014-03-20en_UK
dc.date.filedepositdate2016-01-26en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorMonaghan, Sean|0000-0002-7692-7756en_UK
local.rioxx.authorThompson, Kimberly|en_UK
local.rioxx.authorAdams, Alexandra|en_UK
local.rioxx.authorKempter, Jolanta|en_UK
local.rioxx.authorBergmann, Sven|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-14en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameMonaghan_et_al-2015-Journal_of_Fish_Diseases.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0140-7775en_UK
Appears in Collections:Aquaculture Journal Articles

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