Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21415
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Characterization of PAMP/PRR interactions in European eel (Anguillaanguilla) macrophage-like primary cell cultures
Authors: Callol, Agnes
Roher, Nerea
Amaro, Carmen
MacKenzie, Simon
Contact Email: simon.mackenzie@stir.ac.uk
Keywords: European eel
Macrophages
Innate immunity
Phagocytosis
Issue Date: Oct-2013
Publisher: Elsevier
Citation: Callol A, Roher N, Amaro C & MacKenzie S (2013) Characterization of PAMP/PRR interactions in European eel (Anguillaanguilla) macrophage-like primary cell cultures, Fish and Shellfish Immunology, 35 (4), pp. 1216-1223.
Abstract: The eel (Anguilla anguilla) has been identified as a vulnerable species with stocks dramatically declining over the past decade. In an effort to support the species from overfishing of wild stocks increased interest in eel aquaculture has been notable. In order to expand the scarce knowledge concerning the biology of this species significant research efforts are required in several fields of biology. The development of cell culture systems to study the immune response is a key step towards an increased understanding of the immune response and to develop resources to support further study in this threatened species. Macrophages are one of the most important effector cells of the innate immune system. The capacity to engulf pathogens and orchestrate the immune response relies on the existence of different surface receptors, such as scavenger receptors and toll-like receptors. We have developed and described an eel macrophage-like invitro model and studied its functional and transcriptomic responses. Macrophage-like cells from both head kidney and purified peripheral blood leukocytes were obtained and phagocytic activity measured for different whole bacteria and yeast. Moreover, based on PAMP-PRR association the innate immune response of both head kidney and PBL derived macrophage-like cells was evaluated against different pathogen-associated molecular patterns (PAMPs). Results highlight that peptidoglycan stimulation strongly induces inflammatory mRNA expression reflected in the up-regulation of pro-inflammatory genes IL1β and IL18 in PBL derived cells whereas IL8 is upregulated in head kidney derived cells. Furthermore TLR2 mRNA abundance is regulated by all stimuli supporting a multifunctional role for this pathogen recognition receptor (PRR) in eel macrophage-like cells.
Type: Journal Article
URI: http://hdl.handle.net/1893/21415
DOI Link: http://dx.doi.org/10.1016/j.fsi.2013.07.037
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Universitat Autonoma de Barcelona
Universitat Autonoma de Barcelona
University of Valencia
Complex Systems

Files in This Item:
File Description SizeFormat 
MacKenzie_Fish & Shellfish Immunology 2013.pdf1.59 MBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.