Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21415
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dc.contributor.authorCallol, Agnesen_UK
dc.contributor.authorRoher, Nereaen_UK
dc.contributor.authorAmaro, Carmenen_UK
dc.contributor.authorMacKenzie, Simonen_UK
dc.date.accessioned2015-01-29T23:23:29Z-
dc.date.available2015-01-29T23:23:29Zen_UK
dc.date.issued2013-10en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21415-
dc.description.abstractThe eel (Anguilla anguilla) has been identified as a vulnerable species with stocks dramatically declining over the past decade. In an effort to support the species from overfishing of wild stocks increased interest in eel aquaculture has been notable. In order to expand the scarce knowledge concerning the biology of this species significant research efforts are required in several fields of biology. The development of cell culture systems to study the immune response is a key step towards an increased understanding of the immune response and to develop resources to support further study in this threatened species. Macrophages are one of the most important effector cells of the innate immune system. The capacity to engulf pathogens and orchestrate the immune response relies on the existence of different surface receptors, such as scavenger receptors and toll-like receptors. We have developed and described an eel macrophage-like invitro model and studied its functional and transcriptomic responses. Macrophage-like cells from both head kidney and purified peripheral blood leukocytes were obtained and phagocytic activity measured for different whole bacteria and yeast. Moreover, based on PAMP-PRR association the innate immune response of both head kidney and PBL derived macrophage-like cells was evaluated against different pathogen-associated molecular patterns (PAMPs). Results highlight that peptidoglycan stimulation strongly induces inflammatory mRNA expression reflected in the up-regulation of pro-inflammatory genes IL1β and IL18 in PBL derived cells whereas IL8 is upregulated in head kidney derived cells. Furthermore TLR2 mRNA abundance is regulated by all stimuli supporting a multifunctional role for this pathogen recognition receptor (PRR) in eel macrophage-like cells.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationCallol A, Roher N, Amaro C & MacKenzie S (2013) Characterization of PAMP/PRR interactions in European eel (Anguillaanguilla) macrophage-like primary cell cultures. Fish and Shellfish Immunology, 35 (4), pp. 1216-1223. https://doi.org/10.1016/j.fsi.2013.07.037en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectEuropean eelen_UK
dc.subjectMacrophagesen_UK
dc.subjectInnate immunityen_UK
dc.subjectPhagocytosisen_UK
dc.titleCharacterization of PAMP/PRR interactions in European eel (Anguillaanguilla) macrophage-like primary cell culturesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate3000-01-01en_UK
dc.rights.embargoreason[MacKenzie_Fish Shellfish Immunology 2013.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.fsi.2013.07.037en_UK
dc.citation.jtitleFish and Shellfish Immunologyen_UK
dc.citation.issn1050-4648en_UK
dc.citation.volume35en_UK
dc.citation.issue4en_UK
dc.citation.spage1216en_UK
dc.citation.epage1223en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailsimon.mackenzie@stir.ac.uken_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversity of Valenciaen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.identifier.isiWOS:000324849800019en_UK
dc.identifier.scopusid2-s2.0-84883560573en_UK
dc.identifier.wtid605583en_UK
dc.contributor.orcid0000-0003-1845-6826en_UK
dc.date.accepted2013-07-24en_UK
dcterms.dateAccepted2013-07-24en_UK
dc.date.filedepositdate2015-01-29en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorCallol, Agnes|en_UK
local.rioxx.authorRoher, Nerea|en_UK
local.rioxx.authorAmaro, Carmen|en_UK
local.rioxx.authorMacKenzie, Simon|0000-0003-1845-6826en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate3000-01-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameMacKenzie_Fish Shellfish Immunology 2013.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1050-4648en_UK
Appears in Collections:Aquaculture Journal Articles

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