|Appears in Collections:||Biological and Environmental Sciences Journal Articles|
|Peer Review Status:||Refereed|
|Title:||An essential role for talin during alpha(M)beta(2)-mediated phagocytosis|
Monkley, Susan J
Critchley, David R
|Citation:||Lim J, Wiedemann A, Tzircotis G, Monkley SJ, Critchley DR & Caron E (2007) An essential role for talin during alpha(M)beta(2)-mediated phagocytosis, Molecular Biology of the Cell, 18 (3), pp. 976-985.|
|Abstract:||The cytoskeletal, actin-binding protein talin has been previously implicated in phagocytosis in Dictyostelium discoideum and mammalian phagocytes. However, its mechanism of action during internalization is not understood. Our data confirm that endogenous talin can occasionally be found at phagosomes forming around IgG- and C3bi-opsonized red blood cells in macrophages. Remarkably, talin knockdown specifically abrogates uptake through complement receptor 3 (CR3, CD11b/CD18, alpha(M)beta(2) integrin) and not through the Fc gamma receptor. We show that talin physically interacts with CR3/alpha(M)beta(2) and that this interaction involves the talin head domain and residues W747 and F754 in the beta(2) integrin cytoplasmic domain. The CR3/alpha(M)beta(2)-talin head interaction controls not only talin recruitment to forming phagosomes but also CR3/alpha(M)beta(2) binding activity, both in macrophages and transfected fibroblasts. However, the talin head domain alone cannot support phagocytosis. Our results establish for the first time at least two distinct roles for talin during CR3/alpha(M)beta(2)-mediated phagocytosis, most noticeably activation of the CR3/alpha(M)beta(2) receptor and phagocytic uptake.|
|Rights:||Publisher allows this work to be made available in this repository. Published by American Society for Cell Biology with the following policy: authors are permitted to post the MBoC PDF of their articles (and/or supplemental material) on their personal websites or in an online institutional repository provided there appears always the proper citation of the manuscript in MBoC and a link to the original publication of the manuscript in MBoC. Mol. Biol. Cell March 1, 2007 vol. 18 no. 3 976-985. Available at: http://www.molbiolcell.org/content/18/3/976.short|
|2007 Lim et al talin CR3.pdf||1.15 MB||Adobe PDF||View/Open|
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