Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21088
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Dissociation of recruitment and activation of the small G-protein Rac during Fcgamma receptor-mediated phagocytosis
Authors: Cougoule, Celine
Hoshino, Saiko
Dart, Anna
Lim, Jenson
Caron, Emmanuelle
Contact Email: jenson.lim@stir.ac.uk
Issue Date: 31-Mar-2006
Publisher: American Society for Biochemistry and Molecular Biology
Citation: Cougoule C, Hoshino S, Dart A, Lim J & Caron E (2006) Dissociation of recruitment and activation of the small G-protein Rac during Fcgamma receptor-mediated phagocytosis, Journal of Biological Chemistry, 281 (13), pp. 8756-8764.
Abstract: Rho-family proteins play a central role in most actin-dependent processes, including the control and maintenance of cell shape, adhesion, motility, and phagocytosis. Activation of these GTP-binding proteins is tightly regulated spatially and temporally; however, very little is known of the mechanisms involved in their recruitment and activation in vivo. Because of its inducible, restricted signaling, phagocytosis offers an ideal physiological system to delineate the pathways linking surface receptors to actin remodeling via Rho GTPases. In this study, we investigated the involvement of early regulators of Fcgamma receptor signaling in Rac recruitment and activation. Using a combination of receptor mutagenesis, cellular, molecular, and pharmacological approaches, we show that Src family and Syk kinases control Rac and Vav function during phagocytosis. Importantly, both the immunoreceptor tyrosine-based activation motif within Fcgamma receptor cytoplasmic domain and Src kinase control the recruitment of Vav and Rac. However, Syk activity is dispensable for Vav and Rac recruitment. Moreover, we show that Rac and Cdc42 activities coordinate F-actin accumulation at nascent phagosomes. Our results provide new insights in the understanding of the spatiotemporal regulation of Rho-family GTPase function, and of Rac in particular, during phagocytosis. We believe they will contribute to a better understanding of more complex cellular processes, such as cell adhesion and migration.
Type: Journal Article
URI: http://hdl.handle.net/1893/21088
DOI Link: http://dx.doi.org/10.1074/jbc.M513731200
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Imperial College London
Imperial College London
Imperial College London
Biological and Environmental Sciences
Imperial College London

Files in This Item:
File Description SizeFormat 
2006 Cougoule et al Fc Rac phagocytosis.pdf689.76 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.