Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21007
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Distinguishing gorilla mitochondrial sequences from nuclear integrations and PCR recombinants: Guidelines for their diagnosis in complex sequence databases
Authors: Anthony, Nicola M
Clifford, Stephen L
Johnson-Bawe, Mireille
Abernethy, Katharine
Bruford, Michael W
Wickings, E Jean
Contact Email: k.a.abernethy@stir.ac.uk
Keywords: Nuclear integrations
Numts
Mitochondrial DNA
HV1
Gorilla
Recombination
Control region
Phylogeography
Issue Date: May-2007
Publisher: Elsevier
Citation: Anthony NM, Clifford SL, Johnson-Bawe M, Abernethy K, Bruford MW & Wickings EJ (2007) Distinguishing gorilla mitochondrial sequences from nuclear integrations and PCR recombinants: Guidelines for their diagnosis in complex sequence databases, Molecular Phylogenetics and Evolution, 43 (2), pp. 553-566.
Abstract: Nuclear integrations of mitochondrial DNA (Numts) are widespread in many taxa and if left undetected can confound phylogeny interpretation and bias estimates of mitochondrial DNA (mtDNA) diversity. This is particularly true in gorillas, where recent studies suggest multiple integrations of the first hypervariable (HV1) domain of the mitochondrial control region. Problems can also arise through the inadvertent incorporation of artifacts produced by in vitro recombination between sequence types during polymerase chain reaction amplification. This issue has attracted little attention yet could potentially exacerbate errors in databases already contaminated by Numts. Using a set of existing diagnostic tools, this study set out to systematically inventory Numts and PCR recombinants in a gorilla HV1 sequence database and address the degree to which existing public databases are contaminated. Phylogenetic analysis revealed three distinct gorilla HV1 Numt groups (I, II, and III) that could be readily differentiated from mtDNA sequences by Numt-specific diagnostic sites and sequence-based motifs. Several instances of genuine recombination were also identified by a suite of detection methods. The location of putative breakpoints was identified by eye and by likelihood analysis. Findings from this study reveal widespread nuclear contamination of gorilla HV1 GenBank databases and underline the importance of recognizing not only Numts but also PCR recombinant artifacts as potential sources of data contamination. Guidelines for the routine identification of Numts and in vitro recombinants are presented and should prove useful in the detection of similar artifacts in other species mtDNA databases.
Type: Journal Article
URI: http://hdl.handle.net/1893/21007
DOI Link: http://dx.doi.org/10.1016/j.ympev.2006.09.013
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Cardiff University
Cardiff University
Centre International de Recherches Médicales de Franceville
Biological and Environmental Sciences
Cardiff University
Centre International de Recherches Médicales de Franceville

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